人二倍体细胞中荧光素标记的促生长α球蛋白的胞饮作用

Pinocytosis of fluorescein-labelled growth-promoting alpha-globulin in human diploid cells.

作者信息

Michl J, Baudysová M

出版信息

Cell Biol Int Rep. 1981 Apr;5(4):381-6. doi: 10.1016/0309-1651(81)90008-4.

Abstract

Fluorescein isothiocyanate conjugated growth-promoting alpha-globulin (FITC-GPAG) binds to human diploid cells LEP 19 at 37 degrees C. After the initial binding step, labelled GPAG is progressively internalized by the cells. FITC-GPAG is present in pinocytic vesicles localized in the perinuclear region of the cells and its pinocytosis occurs without cell surface redistribution of GPAG.

摘要

异硫氰酸荧光素偶联的促生长α球蛋白（FITC-GPAG）在37℃时与人二倍体细胞LEP 19结合。初始结合步骤后，标记的GPAG逐渐被细胞内化。FITC-GPAG存在于位于细胞周核区域的胞饮小泡中，其胞饮作用发生时GPAG在细胞表面没有重新分布。