Effect of growth-promoting alpha-globulin on the incorporation of exogenous DNA into L cells. II. Fate of exogenous 3H-DNA after incorporation into recipient cells.

GPAG stimulates the uptake of exogenous 3H-DNA by L cells and facilitates its retention in host cell nuclei during 48-h postcultivation; the nuclei contain 1.5 times more radioactivity than GPAG-free controls. Owing to the well-known properties of GPAG, the formation of the 3H-DNA-GPAG complex may affect the incorporation of exogenous 3-H-DNA and its intracellular transport and retention in the host cell nuclei.