Eade O E, Andre-Ukena S S, Beeken W L
Digestion. 1981;21(1):25-32. doi: 10.1159/000198516.
Mechanical, enzymatic and chelating methods for isolating rat intestinal epithelial cells were compared to determine the best technique for obtaining high yields of viable cells. The mechanical techniques resulted in consistently poor viability compared with the other methods. There was excellent agreement between viability determinations made by trypan blue exclusion and by cytofluorochromasia. Incubation with EDTA, citrate and dithiothreitol, combined with mechanical extrusion of dissociated cells provided the highest yields of viable cells in these studies.
为确定获得高产量活细胞的最佳技术，对分离大鼠肠上皮细胞的机械、酶解和螯合方法进行了比较。与其他方法相比，机械技术导致的细胞活力始终较差。台盼蓝排斥法和细胞荧光染色法测定的细胞活力结果高度一致。在这些研究中，用乙二胺四乙酸（EDTA）、柠檬酸盐和二硫苏糖醇孵育，结合对解离细胞的机械挤压，可获得最高产量的活细胞。
