Kremski V C, Varani L, DeSaive C, Miller P, Nicolini C
J Histochem Cytochem. 1977 Jul;25(7):554-9. doi: 10.1177/25.7.894003.
A successful method has been developed for isolating viable single cell suspensions of intestinal crypt cells from the small bowel of the mouse. The lumen of the intestine was perfused with a 0.2% trypsin solution that dissociated the lining epithelial cells. Crypt cell isolation, which proves to be extremely critical, occurred under optimal mechanical and chemical configurations about 75 min after the beginning of the procedure.
已开发出一种成功的方法，用于从小鼠小肠中分离出具有活性的肠隐窝细胞单细胞悬液。用0.2%的胰蛋白酶溶液灌注肠腔，以使内衬上皮细胞解离。隐窝细胞的分离在该程序开始约75分钟后，在最佳的机械和化学条件下进行，这一过程被证明极为关键。
