一种从人结肠中分离肿瘤组织的快速简便方法。
A rapid and simple procedure for dissociation of tumor tissue from the human colon.
作者信息
Kemmner W, Schlag P, Brossmer R
出版信息
J Cancer Res Clin Oncol. 1987;113(4):400-1. doi: 10.1007/BF00397727.
Abstract
A rapid procedure for dissociation of colon tumor tissue which combines enzymatic and mechanical methods is described. Using this method a cell suspension almost free of cell aggregates is obtained which is further characterized by high cell yield and excellent cell viability.
摘要
本文描述了一种结合酶解法和机械法快速解离结肠肿瘤组织的方法。使用该方法可获得几乎不含细胞聚集体的细胞悬液，其具有高细胞产量和优异细胞活力的特点。
相似文献
1
A rapid and simple procedure for dissociation of tumor tissue from the human colon.一种从人结肠中分离肿瘤组织的快速简便方法。
J Cancer Res Clin Oncol. 1987;113(4):400-1. doi: 10.1007/BF00397727.
2
OncoE25: an AI model for predicting postoperative prognosis in early-onset stage I-III colon and rectal cancer-a population-based study using SEER with dual-center cohort validation.OncoE25：一种用于预测早期I-III期结肠癌和直肠癌术后预后的人工智能模型——一项基于人群的研究，使用监测、流行病学和最终结果（SEER）数据库并进行双中心队列验证
J Transl Med. 2025 Jun 22;23(1):695. doi: 10.1186/s12967-025-06663-4.
3
[Evidence for the extent and oncological benefits of lymphadenectomy in colon and rectal cancer : A narrative review based on meta-analyses].[结肠癌和直肠癌淋巴结清扫范围及其肿瘤学获益的证据：基于荟萃分析的叙述性综述]
Chirurgie (Heidelb). 2025 Apr;96(4):293-305. doi: 10.1007/s00104-024-02212-9. Epub 2025 Jan 10.
4
Activity of cancer chemotherapeutic agents against human colorectal carcinomas grown as primary tissue culture.癌症化疗药物对作为原代组织培养生长的人类结直肠癌的活性。
J Cancer Res Clin Oncol. 1987;113(1):20-6. doi: 10.1007/BF00389962.
5
[Volume and health outcomes: evidence from systematic reviews and from evaluation of Italian hospital data].[容量与健康结果：来自系统评价和意大利医院数据评估的证据]
Epidemiol Prev. 2013 Mar-Jun;37(2-3 Suppl 2):1-100.
6
Strategies for detecting colon cancer in patients with inflammatory bowel disease.炎症性肠病患者结肠癌的检测策略。
Cochrane Database Syst Rev. 2017 Sep 18;9(9):CD000279. doi: 10.1002/14651858.CD000279.pub4.
7
Strategies for detecting colon cancer and/or dysplasia in patients with inflammatory bowel disease.检测炎症性肠病患者结肠癌和/或发育异常的策略。
Cochrane Database Syst Rev. 2006 Apr 19(2):CD000279. doi: 10.1002/14651858.CD000279.pub3.
8
Strategies for detecting colon cancer and/or dysplasia in patients with inflammatory bowel disease.检测炎症性肠病患者结肠癌和/或发育异常的策略。
Cochrane Database Syst Rev. 2004(2):CD000279. doi: 10.1002/14651858.CD000279.pub2.
9
Effects of 3-Bromo-2-oxopropionic acid and LiCl treatment combined with electroporation on apoptotic and metabolic responses in DLD-1 colon cancer cells.3-溴-2-氧代丙酸和氯化锂处理联合电穿孔对DLD-1结肠癌细胞凋亡和代谢反应的影响
Med Oncol. 2025 Jul 12;42(8):326. doi: 10.1007/s12032-025-02898-9.
10
Can a Liquid Biopsy Detect Circulating Tumor DNA With Low-passage Whole-genome Sequencing in Patients With a Sarcoma? A Pilot Evaluation.液体活检能否通过低深度全基因组测序检测肉瘤患者的循环肿瘤DNA？一项初步评估。
Clin Orthop Relat Res. 2025 Jan 1;483(1):39-48. doi: 10.1097/CORR.0000000000003161. Epub 2024 Jun 21.
引用本文的文献
1
A recombinant anti-carcinoembryonic antigen immunoreceptor with combined CD3zeta-CD28 signalling targets T cells from colorectal cancer patients against their tumour cells.一种具有CD3ζ-CD28联合信号传导的重组抗癌胚抗原免疫受体可靶向来自结直肠癌患者的T细胞，使其对抗自身肿瘤细胞。
Gut. 2006 Aug;55(8):1156-64. doi: 10.1136/gut.2005.076208. Epub 2005 Sep 27.
2
Interleukin-12 inhibits tumor growth in a novel angiogenesis canine hemangiosarcoma xenograft model.白细胞介素-12在一种新型血管生成犬血管肉瘤异种移植模型中抑制肿瘤生长。
Neoplasia. 2004 Mar-Apr;6(2):106-16. doi: 10.1593/neo.03334.
3
FasL is more frequently expressed in liver metastases of colorectal cancer than in matched primary carcinomas.FasL在结直肠癌肝转移灶中的表达比配对的原发性癌更频繁。
Br J Cancer. 1999 Mar;79(7-8):1262-9. doi: 10.1038/sj.bjc.6690202.
4
Importance of orthotopic implantation for human tumors as model systems: relevance to metastasis and invasion.原位植入作为人类肿瘤模型系统的重要性：与转移和侵袭的相关性。
Clin Exp Metastasis. 1993 Jan;11(1):5-14. doi: 10.1007/BF00880061.
5
Different sialyltransferase activities in human colorectal carcinoma cells from surgical specimens detected by specific glycoprotein and glycolipid acceptors.通过特异性糖蛋白和糖脂受体检测手术标本中人类结肠癌细胞的不同唾液酸转移酶活性。
Clin Exp Metastasis. 1994 May;12(3):245-54. doi: 10.1007/BF01753893.
本文引用的文献
1
2
Tissue culture of human epithelial cells from benign colonic tumors.良性结肠肿瘤来源的人上皮细胞的组织培养。
In Vitro. 1981 Jul;17(7):632-44. doi: 10.1007/BF02618462.
3
4
Lectin-like activities associated with human and murine neoplastic cells.与人类和鼠类肿瘤细胞相关的凝集素样活性。
Cancer Res. 1981 Sep;41(9 Pt 1):3642-7.
5
Comparative viabilities of rat intestinal epithelial cells prepared by mechanical, enzymatic and chelating methods.通过机械、酶解和螯合方法制备的大鼠肠上皮细胞的比较活力。
Digestion. 1981;21(1):25-32. doi: 10.1159/000198516.
6
Inverse correlation between cell-surface adhesiveness and malignancy in mouse fibroblastoid cell lines.小鼠成纤维样细胞系中细胞表面黏附性与恶性程度之间的负相关。
Int J Cancer. 1979 Mar 15;23(3):392-6. doi: 10.1002/ijc.2910230318.
Zotero 插件