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本文引用的文献

1
Initiation and characterization of cultures of human colonic carcinoma with different biological characteristics utilizing feeder layers of confluent fibroblasts.利用汇合的成纤维细胞饲养层启动具有不同生物学特性的人结肠癌培养物并进行特性鉴定。
Oncodev Biol Med. 1981;2(5):355-66.
2
Tissue culture of human epithelial cells from benign colonic tumors.良性结肠肿瘤来源的人上皮细胞的组织培养。
In Vitro. 1981 Jul;17(7):632-44. doi: 10.1007/BF02618462.
3
Growth state-specific responsiveness of primary cultures of a nude mouse-xenografted human colon carcinoma to 4'-deoxydoxorubicin and a crude human leukocyte alpha-interferon preparation.裸鼠异种移植人结肠癌原代培养物对4'-脱氧阿霉素和粗制人白细胞α-干扰素制剂的生长状态特异性反应性。
Cancer Res. 1982 Sep;42(9):3789-92.
4
Lectin-like activities associated with human and murine neoplastic cells.与人类和鼠类肿瘤细胞相关的凝集素样活性。
Cancer Res. 1981 Sep;41(9 Pt 1):3642-7.
5
Comparative viabilities of rat intestinal epithelial cells prepared by mechanical, enzymatic and chelating methods.通过机械、酶解和螯合方法制备的大鼠肠上皮细胞的比较活力。
Digestion. 1981;21(1):25-32. doi: 10.1159/000198516.
6
Inverse correlation between cell-surface adhesiveness and malignancy in mouse fibroblastoid cell lines.小鼠成纤维样细胞系中细胞表面黏附性与恶性程度之间的负相关。
Int J Cancer. 1979 Mar 15;23(3):392-6. doi: 10.1002/ijc.2910230318.

一种从人结肠中分离肿瘤组织的快速简便方法。

A rapid and simple procedure for dissociation of tumor tissue from the human colon.

作者信息

Kemmner W, Schlag P, Brossmer R

出版信息

J Cancer Res Clin Oncol. 1987;113(4):400-1. doi: 10.1007/BF00397727.

DOI:10.1007/BF00397727
PMID:3597525
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12248398/
Abstract

A rapid procedure for dissociation of colon tumor tissue which combines enzymatic and mechanical methods is described. Using this method a cell suspension almost free of cell aggregates is obtained which is further characterized by high cell yield and excellent cell viability.

摘要

本文描述了一种结合酶解法和机械法快速解离结肠肿瘤组织的方法。使用该方法可获得几乎不含细胞聚集体的细胞悬液，其具有高细胞产量和优异细胞活力的特点。