Quatacker J
Histochem J. 1981 Jan;13(1):109-24. doi: 10.1007/BF01005844.
Adrenergic neurons from the superior cervical ganglion of the rat have been studied with the chromaffin reaction and the zinc iodide-osmium tetroxide method. Phosphotungstic acid staining at a low pH and a combined acid phosphatase reaction and phosphotungstic acid staining have also been performed on glycolmethacrylate-embedded tissue. The results indicate that phosphotungstic acid-positive elements lacking acid phosphatase activity are present at the inner side of the Golgi apparatus. These elements give rise directly to reticular differentiations, carrying catecholamines, or to tubular extensions, representing the origin of the axonal reticulum. On these tubules, reticular differentiations can again be formed at any level. In the cell body, the differentiations are mainly found close to the neurolemma. In the axons, they are especially abundant at the axon terminals. Large granules may be associated with the reticular differentiations and small and large granules may detach from them. It is concluded that the whole catecholamine-producing and/or -storing system in sympathetic neurons can be considered as a direct extension of the Golgi apparatus, set up for local catecholamine synthesis. The relative importance of small and large granules along this system may reflect th functional status of the nerve cell.
运用嗜铬反应和碘化锌 - 四氧化锇法对大鼠颈上神经节的肾上腺素能神经元进行了研究。还对乙二醇甲基丙烯酸酯包埋的组织进行了低pH值的磷钨酸染色以及酸性磷酸酶反应与磷钨酸染色相结合的操作。结果表明，在高尔基体内侧存在缺乏酸性磷酸酶活性的磷钨酸阳性成分。这些成分直接产生携带儿茶酚胺的网状分化结构，或形成代表轴突网状结构起源的管状延伸部分。在这些小管上，网状分化结构可在任何水平再次形成。在细胞体中，分化结构主要见于靠近神经膜处。在轴突中，它们在轴突终末尤其丰富。大颗粒可能与网状分化结构相关联，小颗粒和大颗粒都可能从它们上面脱离。得出的结论是，交感神经元中整个儿茶酚胺产生和/或储存系统可被视为高尔基体的直接延伸，用于局部儿茶酚胺合成。沿着该系统小颗粒和大颗粒的相对重要性可能反映神经细胞的功能状态。
