一种通过具有5'-末端三磷酸基团的分子富集RNA的化学方法。
A chemical method to enrich RNA by molecules having 5'-terminal triphosphate groups.
作者信息
Chumakov P M, Grachev M A, Netesov S V, Shatsky I N
出版信息
Nucleic Acids Res. 1981 Mar 25;9(6):1519-30. doi: 10.1093/nar/9.6.1519.
Abstract
A method is proposed to enrich RNA molecules having a 5'-terminal triphosphate group. The method is based upon selective chemical modification of 5'-triphosphate groups by an antigen-containing amine followed by affinity chromatography on an absorbent loaded with antibodies specific to this antigen. A purification factor up to 37 may be achieved.
摘要
提出了一种富集具有5'-末端三磷酸基团的RNA分子的方法。该方法基于含抗原的胺对5'-三磷酸基团的选择性化学修饰，然后在负载有针对该抗原的特异性抗体的吸附剂上进行亲和色谱。可实现高达37的纯化因子。
相似文献
1
A chemical method to enrich RNA by molecules having 5'-terminal triphosphate groups.一种通过具有5'-末端三磷酸基团的分子富集RNA的化学方法。
Nucleic Acids Res. 1981 Mar 25;9(6):1519-30. doi: 10.1093/nar/9.6.1519.
2
Assay of adenylyl cyclase catalytic activity.腺苷酸环化酶催化活性测定
Methods Enzymol. 1991;195:3-21. doi: 10.1016/0076-6879(91)95150-i.
3
Determination of the molar concentration of messenger RNA.信使核糖核酸摩尔浓度的测定。
Methods Enzymol. 1987;152:262-6. doi: 10.1016/0076-6879(87)52029-8.
4
[Methods of preparation of P-32-labeled nucleosidetriphosphates in alpha and gamma position].[α和γ位P-32标记的核苷三磷酸的制备方法]
Postepy Biochem. 1983;29(3-4):377-92.
5
32P labeling of nonnucleosidic moieties 5'-attached to oligonucleotides.与寡核苷酸5'-连接的非核苷部分的32P标记。
Anal Biochem. 1993 Oct;214(1):338-40. doi: 10.1006/abio.1993.1501.
6
Assay of guanylyl cyclase catalytic activity.鸟苷酸环化酶催化活性测定
Methods Enzymol. 1991;195:345-55. doi: 10.1016/0076-6879(91)95179-n.
7
Ultraviolet crosslinking of DNA-protein complexes via 8-azidoadenine.通过8-叠氮腺嘌呤对DNA-蛋白质复合物进行紫外线交联。
Methods Mol Biol. 2001;148:323-35. doi: 10.1385/1-59259-208-2:323.
8
Sensitive radiosubstrate/high-performance liquid chromatography assays for flavocoenzyme-forming enzymes.用于黄素辅酶形成酶的灵敏放射性底物/高效液相色谱分析方法
Methods Enzymol. 1986;122:237-9. doi: 10.1016/0076-6879(86)22176-x.
9
An in vitro transcription system from BmN cells of the silkworm, Bombyx mori.来自家蚕BmN细胞的体外转录系统。
Methods Mol Biol. 1995;37:233-44. doi: 10.1385/0-89603-288-4:233.
10
Mapping of gene transcripts by nuclease protection assays and cDNA primer extension.通过核酸酶保护分析和cDNA引物延伸法对基因转录本进行定位。
Methods Enzymol. 1987;152:611-32. doi: 10.1016/0076-6879(87)52069-9.
本文引用的文献
1
[Gamma-anilides of nucleoside-5'triphosphates as substrates of DNA-dependent RNA-polymerase of Escherichia coli].[核苷-5'-三磷酸的γ-苯胺化物作为大肠杆菌DNA依赖性RNA聚合酶的底物]
Mol Biol (Mosk). 1980 May-Jun;14(3):575-85.
2
[Structure of nuclear pre-mRNA. XIII. Hybridization properties of triphosphorylated 5'-end fragments].[核内前体信使核糖核酸的结构。十三。三磷酸化5′末端片段的杂交特性]
Mol Biol (Mosk). 1980;14(1):212-22.
3
Studies on some interactions and reactions of oligonucleotides in aqueous solution.寡核苷酸在水溶液中的一些相互作用和反应的研究。
Biochemistry. 1966 Aug;5(8):2722-8. doi: 10.1021/bi00872a032.
4
The properties of oligonucleotide fragments containing the triphosphorylated 5'-termini of nuclear pre-mRNA from Ehrlich ascites carcinoma cells.来自艾氏腹水癌细胞的核内前体信使核糖核酸中含有三磷酸化5′末端的寡核苷酸片段的性质。
Mol Biol Rep. 1976 Apr;2(5):369-77. doi: 10.1007/BF00366258.
5
Nucleotide sequence of bacteriophage phi X174 DNA.噬菌体φX174 DNA的核苷酸序列。
Nature. 1977 Feb 24;265(5596):687-95. doi: 10.1038/265687a0.
6
7
8
Localization of the 3' end of Escherichia coli 16 S RNA by electron microscopy of antibody-labelled subunits.通过抗体标记亚基的电子显微镜观察对大肠杆菌16S RNA 3'末端进行定位
J Mol Biol. 1979 Oct 9;133(4):501-15. doi: 10.1016/0022-2836(79)90404-2.
9
A rightward promoter to the left of the att site of lambda phage DNA: possible participant in site-specific recombination.位于λ噬菌体DNA附着位点左侧的向右启动子：可能参与位点特异性重组。
Gene. 1979 Nov;7(3-4):181-95. doi: 10.1016/0378-1119(79)90045-3.
Zotero 插件