Martinez-Alonso C, Coutinho A, von Boehmer H, Bernabé R
Eur J Immunol. 1981 Mar;11(3):172-4. doi: 10.1002/eji.1830110303.
Helper cells, with specificity for the haptens (4-hydroxy-3-nitro-phenyl)acetyl (NP) or (4-hydroxy-5-iodo-3-nitro-phenyl)acetyl (NIP), were raised in B10.BR mice by in vivo priming and in vitro long-term enrichment with hapten-derivatized syngeneic spleen cells. Upon co-culture with the homologous antigen (NP or NIP self), selected helper cells specifically responded by proliferation and by inducing large numbers of B cells to clonal expansion and immunoglobulin secretion. Criss-cross experiments demonstrated the nonheteroclitic nature of antigen recognition by helper cells, as the proliferative and helper cell activities were in every case one order of magnitude higher when confronted with the homologous hapten used for immunization.
通过体内致敏和用半抗原衍生的同基因脾细胞进行体外长期富集，在B10.BR小鼠中产生了对半抗原（4-羟基-3-硝基苯基）乙酰基（NP）或（4-羟基-5-碘-3-硝基苯基）乙酰基（NIP）具有特异性的辅助细胞。与同源抗原（NP或NIP自身）共培养时，所选辅助细胞通过增殖以及诱导大量B细胞进行克隆扩增和免疫球蛋白分泌而产生特异性反应。交叉实验证明了辅助细胞抗原识别的非交叉反应性，因为在每种情况下，当面对用于免疫的同源半抗原时，增殖和辅助细胞活性都高一个数量级。
