Bikoff E
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4156-60. doi: 10.1073/pnas.79.13.4156.
(4-Hydroxy-3-nitrophenyl)acetyl (NP)-modified BALB/c immunoglobulins were used as the immunogen for induction of a proliferative response in BALB/c mice. As is true for responses to other soluble antigens, proliferation was dependent on Lyt-1 cells and histocompatible radioresistant accessory cells. Lyt-1 cells directed against NP-modified self immunoglobulin are specific for the immunizing hapten NP. However, they do not recognize hapten per se. Rather, they see complex determinants comprised of both the hapten NP and the immunoglobulin self-carrier. Distinct specificities were created by coupling the same hapten to different monoclonal BALB/c antibody molecules or by attaching the hapten to the immunoglobulin self-carrier via a spacer molecule. It is proposed that determinants created by attaching haptens to self-immunoglobulin molecules are similar to those recognized by anti-idiotypic T cells.
(4-羟基-3-硝基苯基)乙酰基(NP)修饰的BALB/c免疫球蛋白被用作免疫原,以诱导BALB/c小鼠产生增殖反应。与对其他可溶性抗原的反应一样,增殖依赖于Lyt-1细胞和组织相容性抗辐射辅助细胞。针对NP修饰的自身免疫球蛋白的Lyt-1细胞对免疫原性半抗原NP具有特异性。然而,它们并不识别半抗原本身。相反,它们识别由半抗原NP和免疫球蛋白自身载体组成的复合决定簇。通过将相同的半抗原偶联到不同的单克隆BALB/c抗体分子上,或通过间隔分子将半抗原连接到免疫球蛋白自身载体上,可产生不同的特异性。有人提出,将半抗原连接到自身免疫球蛋白分子上所产生的决定簇与抗独特型T细胞所识别的决定簇相似。