Ting S, Zweiman B, Lavker R, Dunsky E H
J Allergy Clin Immunol. 1981 Jul;68(1):65-71. doi: 10.1016/0091-6749(81)90125-1.
Although it has been shown that histamine inhibits antigen-induced in vitro histamine release from basophils, it is unclear whether histamine inhibits in vivo mediator release in human allergic reactions. We report effects of exogenous histamine on histamine release and inflammatory cell responses in antigen-challenged skin sites in eight ragweed-sensitive individuals. Four heat-suction blisters in each subject were unroofed, and a collection chamber was appended to each blister base. Chamber A contained 1000 PNU/ml ragweed extract; chamber B contained buffered saline (control fluid); chamber C contained 1000 PNU/ml ragweed and 50 ng (5 x 10(-7) M) of histamine; and chamber D contained histamine alone (50 ng). Comparative analyses of chamber histamine levels in individual subjects showed that (1) histamine levels in chamber A were significantly greater than those in chamber B (p less than 0.01) and that histamine levels in chamber C were not significantly different than those in chamber D (p less than 0.5). Likewise, comparison of eosinophils attaching to membrane filters appended to the chamber bases for 2 hr showed that there were significantly more eosinophils in chamber A than in chamber B (p less than 0.01) and that there was no significant difference in eosinophil numbers on filters appended to chamber C vs chamber D. In three of four subjects studied, addition of exogenous histamine (50 ng/ml) to ragweed before intradermal injection inhibited the ultrastructural mast cell alterations seen within 10 min after injection of ragweed alone. In the one subject in which mast cell alterations were not prevented, exogenous histamine also did not inhibit antigen-induced histamine release or subsequent eosinophil accumulation in the skin chambers.
尽管已有研究表明组胺可抑制抗原诱导的嗜碱性粒细胞体外组胺释放，但组胺是否能抑制人体过敏反应中的体内介质释放尚不清楚。我们报告了外源性组胺对8名豚草敏感个体抗原激发皮肤部位组胺释放和炎症细胞反应的影响。给每个受试者的4个热吸疱去除疱顶，并在每个疱底部附加一个收集室。A室含有1000 PNU/ml豚草提取物；B室含有缓冲盐水（对照液）；C室含有1000 PNU/ml豚草和50 ng（5×10⁻⁷ M）组胺；D室仅含有组胺（50 ng）。对个体受试者各室组胺水平的比较分析表明：（1）A室组胺水平显著高于B室（p＜0.01），且C室组胺水平与D室无显著差异（p＜0.5）。同样，对附着在室底部膜滤器上2小时的嗜酸性粒细胞进行比较，发现A室的嗜酸性粒细胞明显多于B室（p＜0.01），且C室和D室滤器上的嗜酸性粒细胞数量无显著差异。在4名受试对象中的3名中，皮内注射豚草前向其中加入外源性组胺（50 ng/ml）可抑制单独注射豚草后10分钟内所见的超微结构肥大细胞改变。在1名肥大细胞改变未被阻止的受试者中，外源性组胺也未抑制抗原诱导的组胺释放或随后皮肤室内嗜酸性粒细胞的积聚。
