Talbot S F, Atkins P C, Valenzano M, Zweiman B
J Allergy Clin Immunol. 1984 Dec;74(6):819-26. doi: 10.1016/0091-6749(84)90185-4.
To evaluate the contribution of mast cell-derived mediators in the late cutaneous allergic response, the duration and quantity of antigen-induced histamine release was compared to the intensity of the antigen-induced skin reactions in atopic volunteers. Chambers containing either pollen extract or buffer were appended to denuded bases for 1 hr and were replaced hourly with buffer for 3 additional hr. These were compared to the extinction dilution skin test titer and to the mean diameters of the 20-minute wheal and induration at 6 and 8 hr after intradermal injection of antigen. Chamber-fluid histamine levels were significantly higher at antigen than at buffer sites throughout the 4 hr. The hourly histamine levels correlated with the size of the induration at 6 and 8 hr but not with the wheal size or skin test titer. We conclude that (1) histamine is released for at least 4 hr at skin sites of antigen challenge as a consequence of prolonged release either from individual or sequentially activated mast cells, and (2) the quantity of histamine released correlates with the intensity of the late-phase skin response. We hypothesize that histamine might be a marker for prolonged release from the mast cell of other mediators that are responsible for the late-phase response.
为评估肥大细胞衍生介质在迟发性皮肤过敏反应中的作用，将抗原诱导的组胺释放的持续时间和量与特应性志愿者中抗原诱导的皮肤反应强度进行比较。将含有花粉提取物或缓冲液的小室附着于裸露的皮肤基底1小时，之后每小时用缓冲液更换一次，持续3小时。将这些结果与皮肤点刺试验效价以及皮内注射抗原后6小时和8小时的20分钟风团和硬结的平均直径进行比较。在整个4小时内，小室液中的组胺水平在抗原部位显著高于缓冲液部位。每小时的组胺水平与6小时和8小时的硬结大小相关，但与风团大小或皮肤试验效价无关。我们得出结论：（1）由于单个或相继激活的肥大细胞的持续释放，组胺在抗原攻击的皮肤部位释放至少4小时；（2）释放的组胺量与迟发性皮肤反应的强度相关。我们推测组胺可能是负责迟发性反应的其他介质从肥大细胞持续释放的一个标志物。
