Eskenasy A
Morphol Embryol (Bucur). 1981 Jan-Mar;27(1):55-61.
In order to specify the ultrastructure of changes induced in the rabbit lung tissue during sensitization to antigens - previously studied by conventional methods -, two groups of rabbits were weekly sensitized by intramuscular injections, the first group with complete Freund adjuvant (CFA) only, the second with CFA to which human gammaglobulin was added. Fragments from caudal lobes harvested six days after the 3rd, 4th and 5th injections were processed for electron microscopy. This investigation confirmed the general process of cell accumulation within alveolar walls, interlobular areas and bronchial subepithelial layers previously appreciated histochemically and morphometrically. The predominating cells in both rabbit groups were histiocytes (macrophages) of local and blood origin; a monocyte marginating an alveolar capillary was evidenced. In the rabbits sensitized with CFA only, histiocytes were largely accompanied by lymphocytes; between them some relationships may be ultrastructurally observed. An increased number of fibrils and fibroblasts was also seen in this group. The animals sensitized with CFA containing human gamma-globulin showed active plasmoblasts and plasma cells among histiocytes; this picture corresponded to the high increase of serum precipitins. These ultrastructural changes form the basis on which hypersensitivity reactions may be challenged experimentally and during human immune chronic lung diseases.
为了明确兔肺组织在抗原致敏过程中所诱导变化的超微结构（此前已用传统方法进行过研究），将两组兔每周进行一次肌肉注射致敏，第一组仅注射完全弗氏佐剂（CFA），第二组注射添加了人丙种球蛋白的CFA。在第3、4和5次注射后6天采集尾叶碎片，进行电子显微镜检查。本研究证实了先前通过组织化学和形态计量学所认识到的肺泡壁、小叶间区域和支气管上皮下层内细胞积聚的一般过程。两组兔中占主导地位的细胞均为局部来源和血液来源的组织细胞（巨噬细胞）；可见一个单核细胞贴附于肺泡毛细血管。在仅用CFA致敏的兔中，组织细胞大量伴有淋巴细胞；在它们之间可通过超微结构观察到一些关系。该组中还可见原纤维和成纤维细胞数量增加。用含人丙种球蛋白的CFA致敏的动物，在组织细胞中可见活跃的成浆细胞和浆细胞；此现象与血清沉淀素的高度增加相符。这些超微结构变化构成了在实验中和人类免疫性慢性肺部疾病期间对超敏反应进行挑战的基础。
