Sharma O K, Goswami B B
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2221-4. doi: 10.1073/pnas.78.4.2221.
Extracts of interferon-treated cells synthesize unique 2',5'-linked oligo(adenylic acid) 5'-phosphates in the presence of ATP and double-stranded RNA. 2',5'-linked oligo(adenylic acid) 5'-triphosphate inhibits protein synthesis at nanomolar concentrations by activating RNase. We have observed that oligo(adenylic acid) 5'-monophosphate and 5'-triphosphate are potent inhibitors of vaccinia mRNA methylation in vitro. Both the methylation of mRNA methylation is not due to degradation of the mRNA. Inhibition of the 5'-terminal guanine at the 7 position and the 2'-O-ribose methylation of the penultimate nucleoside are inhibited. Such inhibition of the requisite modification of the 5' terminus of mRNA by 2',5'-linked oligo(adenylic acids) may be a mechanism of interferon action against both DNA and RNA viruses in which mRNAs derived from them are capped.
在ATP和双链RNA存在的情况下,经干扰素处理的细胞提取物会合成独特的2',5'-连接的寡聚(腺苷酸)5'-磷酸。2',5'-连接的寡聚(腺苷酸)5'-三磷酸通过激活核糖核酸酶在纳摩尔浓度下抑制蛋白质合成。我们观察到,寡聚(腺苷酸)5'-单磷酸和5'-三磷酸在体外是痘苗病毒mRNA甲基化的有效抑制剂。mRNA甲基化的抑制并非由于mRNA的降解。5'-末端鸟嘌呤在第7位的甲基化以及倒数第二个核苷的2'-O-核糖甲基化均受到抑制。2',5'-连接的寡聚(腺苷酸)对mRNA 5'末端必需修饰的这种抑制作用可能是干扰素针对DNA和RNA病毒发挥作用的一种机制,这些病毒产生的mRNA带有帽结构。