• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

同源干扰素处理的痘苗病毒感染鸡胚成纤维细胞中的RNA甲基化

RNA methylation in vaccinia virus-infected chick embryo fibroblasts treated with homologous interferon.

作者信息

Kroath H, Gross H J, Jungwirth C, Bodo G

出版信息

Nucleic Acids Res. 1978 Jul;5(7):2441-54. doi: 10.1093/nar/5.7.2441.

DOI:10.1093/nar/5.7.2441
PMID:673858
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342175/
Abstract

Interferon-pretreatment of vaccinia-infected chick embryo fibroblasts resulted in a greater than 50% decrease in ribose methylation of the penultimate "cap" nucleotide in virus-specific mRNA. However, in contrast to results obtained with cell-free systems, in intact infected cells there was (a) no detectable reduction in methylation of the 5'-ultimate m7G of viral mRNA; (b) a virus specificity of the interferon-induced change in mRNA "CAP"-methylation seems unlikely and (c) analysis of the ribosomal and transfer RNA fractions isolated from interferon-treated and control cells revealed identical patterns of methylated nucleotides. Thus, the interferon-induced change in methylation is specific for mRNA "CAPS".

摘要

用干扰素预处理感染痘苗病毒的鸡胚成纤维细胞，会使病毒特异性mRNA中倒数第二个“帽”核苷酸的核糖甲基化减少50%以上。然而，与无细胞系统得到的结果相反，在完整的感染细胞中：(a) 病毒mRNA的5'-末端m7G甲基化没有可检测到的减少；(b) 干扰素诱导的mRNA“帽”甲基化变化似乎没有病毒特异性；(c) 对从干扰素处理组和对照组细胞中分离出的核糖体RNA和转运RNA组分的分析显示，甲基化核苷酸的模式相同。因此，干扰素诱导的甲基化变化对mRNA“帽”是特异性的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f22d/342175/dea820432432/nar00468-0233-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f22d/342175/24d44d4782f2/nar00468-0230-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f22d/342175/ecbadff4c5fd/nar00468-0231-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f22d/342175/4001ee1064bd/nar00468-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f22d/342175/dea820432432/nar00468-0233-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f22d/342175/24d44d4782f2/nar00468-0230-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f22d/342175/ecbadff4c5fd/nar00468-0231-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f22d/342175/4001ee1064bd/nar00468-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f22d/342175/dea820432432/nar00468-0233-b.jpg

相似文献

1
RNA methylation in vaccinia virus-infected chick embryo fibroblasts treated with homologous interferon.同源干扰素处理的痘苗病毒感染鸡胚成纤维细胞中的RNA甲基化
Nucleic Acids Res. 1978 Jul;5(7):2441-54. doi: 10.1093/nar/5.7.2441.
2
Increased turnover of vaccinia virus-specific immediate early RNAs in interferon-treated chick embryo fibroblasts.在干扰素处理的鸡胚成纤维细胞中牛痘病毒特异性立即早期RNA的周转率增加。
Immunobiology. 1987 Sep;175(3):195-201. doi: 10.1016/S0171-2985(87)80028-1.
3
Reduced steady-state levels of vaccinia virus-specific early mRNAs in interferon-treated chick embryo fibroblasts.在经干扰素处理的鸡胚成纤维细胞中，牛痘病毒特异性早期信使核糖核酸的稳态水平降低。
Virology. 1987 May;158(1):28-33. doi: 10.1016/0042-6822(87)90234-0.
4
Methylation of vaccinia virus-specific mRNA in the interferon-treated chick cell.干扰素处理的鸡细胞中痘苗病毒特异性mRNA的甲基化
Virology. 1979 Jan 30;92(2):572-7. doi: 10.1016/0042-6822(79)90160-0.
5
Expression of authentic vaccinia virus-specific and inserted viral and cellular genes under control of an early vaccinia virus promoter is regulated post-transcriptionally in interferon-treated chick embryo fibroblasts.在经干扰素处理的鸡胚成纤维细胞中，早期牛痘病毒启动子控制下的正宗牛痘病毒特异性基因以及插入的病毒和细胞基因的表达在转录后受到调控。
Virology. 1992 May;188(1):114-21. doi: 10.1016/0042-6822(92)90740-g.
6
Inhibition of vaccinia mRNA methylation by 2',5'-linked oligo(adenylic acid) triphosphate.2',5'-连接的三磷酸寡聚腺苷酸对痘苗病毒mRNA甲基化的抑制作用。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2221-4. doi: 10.1073/pnas.78.4.2221.
7
Inhibition of early vaccinia virus ribonucleic acid synthesis in interferon-treated chicken embryo fibroblasts.干扰素处理的鸡胚成纤维细胞中早期痘苗病毒核糖核酸合成的抑制作用
J Virol. 1972 Feb;9(2):286-9. doi: 10.1128/JVI.9.2.286-289.1972.
8
Increased methylation of RNA in SV40-infected interferon-treated cells.在感染SV40的经干扰素处理的细胞中RNA甲基化增加。
Virology. 1981 Jul 15;112(1):109-18. doi: 10.1016/0042-6822(81)90617-6.
9
Effect of interferon on integrity of vaccinia virus and ribosomal RNA in infected cells.干扰素对感染细胞中痘苗病毒完整性和核糖体RNA的影响。
Virology. 1984 Apr 15;134(1):40-51. doi: 10.1016/0042-6822(84)90270-8.
10
Impairment of reovirus mRNA 'cap' methylation in interferon-treated mouse L929 cells.呼肠孤病毒mRNA“帽”甲基化在干扰素处理的小鼠L929细胞中的损伤
Biochim Biophys Acta. 1979 May 24;562(3):471-80. doi: 10.1016/0005-2787(79)90110-2.

引用本文的文献

1
The interferon renaissance: molecular aspects of induction and action.干扰素的复兴：诱导与作用的分子层面
Microbiol Rev. 1981 Jun;45(2):244-66. doi: 10.1128/mr.45.2.244-266.1981.
2
Inhibition of vaccinia mRNA methylation by 2',5'-linked oligo(adenylic acid) triphosphate.2',5'-连接的三磷酸寡聚腺苷酸对痘苗病毒mRNA甲基化的抑制作用。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2221-4. doi: 10.1073/pnas.78.4.2221.
3
Enhanced therapeutic efficacy of poly(ICLC) and ribavirin combinations against Rift Valley fever virus infection in mice.

本文引用的文献

1
The purification fo four strains of poxvirus.四种痘病毒株的纯化
Virology. 1962 Sep;18:9-18. doi: 10.1016/0042-6822(62)90172-1.
2
Methyl-deficient mammalian transfer RNA: II. Homologous methylation in vitro of liver tRNA from normal and ethionine-fed rats: ethionine effect on 5-methyl-cytidine synthesis in vivo.甲基缺乏的哺乳动物转运RNA：II. 正常和乙硫氨酸喂养大鼠肝脏tRNA的体外同源甲基化：乙硫氨酸对体内5-甲基胞嘧啶合成的影响
Nucleic Acids Res. 1974 Oct;1(10):1259-67. doi: 10.1093/nar/1.10.1259.
3
Acrylamide gel electrophoresis of HeLa cell nucleolar RNA.
聚肌胞苷酸（聚肌苷酸-聚胞苷酸，poly(ICLC)）与利巴韦林联合用药对小鼠裂谷热病毒感染的治疗效果增强。
Antimicrob Agents Chemother. 1987 Jul;31(7):986-90. doi: 10.1128/AAC.31.7.986.
4
Altered or increased transfer-RNA methylation in the course of Interferon action on cells in culture?在干扰素作用于培养细胞的过程中，转运核糖核酸甲基化是否发生改变或增加？
Experientia. 1979 May 15;35(5):576-8. doi: 10.1007/BF01960327.
人宫颈癌细胞核仁RNA的聚丙烯酰胺凝胶电泳
Proc Natl Acad Sci U S A. 1967 Sep;58(3):1088-95. doi: 10.1073/pnas.58.3.1088.
4
The synthesis of poxvirus-specific RNA in interferon-treated cells.干扰素处理的细胞中痘病毒特异性RNA的合成。
Virology. 1972 Apr;48(1):59-70. doi: 10.1016/0042-6822(72)90114-6.
5
Protein synthesis in pox-infected cells treated with interferon.用干扰素处理的痘病毒感染细胞中的蛋白质合成。
Virology. 1972 Oct;50(1):140-7. doi: 10.1016/0042-6822(72)90354-6.
6
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.通过寡聚胸苷酸纤维素柱层析法纯化具有生物活性的珠蛋白信使核糖核酸。
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12. doi: 10.1073/pnas.69.6.1408.
7
Release of the inhibition of messenger RNA translation in extracts of interferon-treated Ehrlich ascites tumor cells by added transfer RNA.通过添加转移RNA来解除干扰素处理的艾氏腹水肿瘤细胞提取物中信使核糖核酸翻译的抑制作用。
Biochem Biophys Res Commun. 1974 Apr 8;57(3):763-70. doi: 10.1016/0006-291x(74)90612-3.
8
Correlation between a specific isoaccepting lysyl transfer ribonucleic acid and cell division in mammalian tissues.哺乳动物组织中一种特定同功接受赖氨酰转移核糖核酸与细胞分裂之间的相关性。
Biochemistry. 1973 Sep 25;12(20):3978-84. doi: 10.1021/bi00744a030.
9
Base analysis of ribopolynucleotides by chemical tritium labeling: a methodological study with model nucleosides and purified tRNA species.通过化学氚标记对核糖多核苷酸进行基础分析：对模型核苷和纯化的tRNA种类的方法学研究。
Anal Biochem. 1972 Jul;48(1):172-98. doi: 10.1016/0003-2697(72)90181-9.
10
Methylation of cytoplasmic adenovirus RNA synthesized early and late in productive infection.在增殖性感染早期和晚期合成的细胞质腺病毒RNA的甲基化。
Biochem Biophys Res Commun. 1975 Sep 2;66(1):67-74. doi: 10.1016/s0006-291x(75)80295-6.