Washburn K W, Maeda Y, Lanza G M
Anim Blood Groups Biochem Genet. 1980;11(4):261-9. doi: 10.1111/j.1365-2052.1980.tb01517.x.
Polymorphism in plasma amylase, plasma alkaline phosphatase, non-specific esterase and red cell esterase-D of the Athens-Canadian randombred (ACRB) population of chickens was determined by polyacrylamide and starch gel electrophoresis. Amylase alleles Amy-1A and Amy-1B were segregating in the ACRB population with frequencies of 0.45 and 0.55 respectively. For the plasma alkaline phosphatase the F and S bands, the B band and a new isozyme migrating at a faster rate than the previously reported F band were detected. A genetic nomenclature for plasma alkaline phosphatase is suggested which considers the difference between the F and S bands as the presence or absence of sialic acid attached to a primary protein. Plasma esterase activity was observed in all four of the regions previously reported, but there was no polymorphism found in any of the loci. All birds in this population showed the same red-cell esterase-D phenotype which consisted of a main band with sub-bands on each side.
通过聚丙烯酰胺和淀粉凝胶电泳测定了雅典-加拿大随机交配（ACRB）鸡群血浆淀粉酶、血浆碱性磷酸酶、非特异性酯酶和红细胞酯酶-D的多态性。淀粉酶等位基因Amy-1A和Amy-1B在ACRB群体中分离，频率分别为0.45和0.55。对于血浆碱性磷酸酶，检测到F和S带、B带以及一条迁移速度比先前报道的F带更快的新同工酶。建议了一种血浆碱性磷酸酶的遗传命名法，该命名法将F和S带之间的差异视为连接在主要蛋白质上的唾液酸的存在或不存在。在先前报道的所有四个区域均观察到血浆酯酶活性，但在任何位点均未发现多态性。该群体中的所有鸡均表现出相同的红细胞酯酶-D表型，该表型由一条主带和两侧的亚带组成。
