Tamaki Y, Morichi T
Anim Blood Groups Biochem Genet. 1976;7(3):179-84. doi: 10.1111/j.1365-2052.1976.tb01392.x.
The effects of urea and heat treatments on electrophoretic pattern and activity were investigated in chicken plasma alkaline phosphatase (AP). The B band, which had a slower migrating rate to the F or S band irrespective of isozyme types, was labile to urea (4M) and heat treatments (60 degrees C, 10 min), while the F and S bands were stable to the same treatments. From these results, the genetic control of the three chicken plasma AP isozymes, i.e., F, S and B bands, was discussed. The total AP activity of the F or S type was little affected by urea treatment in spite of the unstableness of the B band. It is considered that the B band inactivated by urea restores the activity when the urea concentration was reduced. The AP activity was reduced by the heat treatment. The reduction may be primarily due to the loss of the activity of the B band.
研究了尿素和热处理对鸡血浆碱性磷酸酶（AP）电泳图谱和活性的影响。无论同工酶类型如何，迁移速率比F带或S带慢的B带对尿素（4M）和热处理（60℃，10分钟）不稳定，而F带和S带对相同处理稳定。根据这些结果，讨论了三种鸡血浆AP同工酶，即F、S和B带的遗传控制。尽管B带不稳定，但F型或S型的总AP活性受尿素处理的影响很小。据认为，被尿素灭活的B带在尿素浓度降低时会恢复活性。热处理使AP活性降低。这种降低可能主要是由于B带活性的丧失。
