恙虫病立克次氏体抗原：通过聚丙烯酰胺凝胶电泳分离并通过酶联免疫吸附测定法鉴定
Antigens of scrub typhus rickettsiae: separation by polyacrylamide gel electrophoresis and identification by enzyme-linked immunosorbent assay.
作者信息
Eisemann C S, Osterman J V
出版信息
Infect Immun. 1981 May;32(2):525-33. doi: 10.1128/iai.32.2.525-533.1981.
Abstract
Antigens of plaque-purified Rickettsia tsutsugamushi strains Gilliam, Karp, and Kato were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and were analyzed by an enzyme-linked immunosorbent assay. Six antigens were identified in each of the three prototype strains; in strain Gilliam, these antigens were located in the cell envelope fraction of the organisms. Reactivity of these isolated antigens with homologous or heterologous immune sera indicated that different macromolecules existed in all three strains, although they exhibited very similar mobilities during electrophoresis. Antigens of strain Gilliam reacted equally well with antibodies directed against Gilliam, Karp, or Kato rickettsiae. However, strains Karp and Kato each had two distinct antigens which did not react with heterologous antisera. R. tsutsugamushi antigens retained immunogenicity after electrophoresis, and antisera raised against them reacted with intact organisms and exhibited specificity in reactions with isolated antigens.
摘要
用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离斑点纯化的恙虫病东方体菌株吉利亚姆株、卡尔普株和加藤株的抗原，并用酶联免疫吸附测定法进行分析。在这三种原型菌株中各鉴定出六种抗原；在吉利亚姆株中，这些抗原位于该生物体的细胞膜部分。这些分离抗原与同源或异源免疫血清的反应性表明，尽管它们在电泳过程中迁移率非常相似，但在所有三种菌株中都存在不同的大分子。吉利亚姆株的抗原与针对吉利亚姆株、卡尔普株或加藤株立克次体的抗体反应同样良好。然而，卡尔普株和加藤株各有两种不同的抗原，它们不与异源抗血清反应。恙虫病东方体抗原在电泳后仍保留免疫原性，针对它们产生的抗血清与完整生物体反应，并在与分离抗原的反应中表现出特异性。
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