Vánky F, Argov S
Int J Cancer. 1980 Oct 15;26(4):405-11. doi: 10.1002/ijc.2910260404.
In a high proportion of cases blood lymphocytes from cancer patients cultured with autologous tumor biopsy cells undergo blastogenesis. In addition, cytotoxicity against autologous tumor cells is generated. These autoactivated lymphocytes also kill the highly NK-sensitive K-562 and Molt-4 cells. Addition of interferon (IF) at the initiation of the cultures inhibited blastogenesis and the generation of autologous cytotoxicity. On the other hand, lymphocytes already activated for autologous killing in the mixed cultures were not affected by short-term IF treatment prior to the cytotoxic assay. Lymphocytes cultured alone lose their cycotoxicity against K-562. However, when IF was present in these cultures the lymphocytes were cytotoxic for K-562. In addition, lymphocytes cultured for 6 days under conditions which did not result in activation killing of Molt-4 cells could be induced by short-term incubation of the effectors with IF.
在很大比例的病例中，与自体肿瘤活检细胞一起培养的癌症患者血液淋巴细胞会发生母细胞化。此外，还会产生针对自体肿瘤细胞的细胞毒性。这些自身激活的淋巴细胞也会杀死对NK高度敏感的K-562和Molt-4细胞。在培养开始时添加干扰素（IF）可抑制母细胞化和自体细胞毒性的产生。另一方面，在混合培养中已经被激活用于自体杀伤的淋巴细胞在细胞毒性测定之前不受短期IF处理的影响。单独培养的淋巴细胞会失去对K-562的细胞毒性。然而，当这些培养物中存在IF时，淋巴细胞对K-562具有细胞毒性。此外，在未导致对Molt-4细胞进行激活杀伤的条件下培养6天的淋巴细胞，可通过效应细胞与IF的短期孵育来诱导其产生细胞毒性。
