Vose B M, Vánky F, Fopp M, Klein E
Int J Cancer. 1978 May 15;21(5):588-93. doi: 10.1002/ijc.2910210508.
Blood lymphocytes from 22 cancer patients were examined for cytotoxicity against autologous tumour cells in a short-term 51Cr release assay. Only three showed reactivity. In an attempt to increase cytotoxic potential in these and induce reactivity in non-reactive cases, the lymphocytes were cultured alone or with autologous tumour cells for 6 days. Upon subsequent testing against frozen, stored targets, nine samples reacted, including two of those with primary reactivity. In seven cases augmented cytotoxicity was evident in mixed cultures compared with lymphocytes cultured alone. Two of 10 cases showed cytotoxicity against the K562 cell line after culture and, in two of 13 tests in which allogeneic tumour biopsy targets were used, weak reactivity was bound. Cytotoxicity for autologous tumour biopsy cells was uniformly accompanied by positive blastogenesis in MLTI assays. In four cases blastogenesis occurred without the induction of cytotoxicity.
采用短期铬-51释放试验，检测了22例癌症患者血液淋巴细胞对自体肿瘤细胞的细胞毒性。只有3例表现出反应性。为了增强这些患者的细胞毒性潜能并诱导无反应病例产生反应，将淋巴细胞单独培养或与自体肿瘤细胞共同培养6天。随后针对冷冻保存的靶细胞进行检测时，9个样本出现反应，其中包括最初有反应的2例。与单独培养的淋巴细胞相比，7例混合培养中细胞毒性增强明显。10例中有2例在培养后对K562细胞系表现出细胞毒性，在13次使用异体肿瘤活检靶细胞的试验中有2次出现微弱反应。对自体肿瘤活检细胞的细胞毒性在混合淋巴细胞肿瘤培养物检测中均伴有阳性母细胞生成。4例出现母细胞生成但未诱导出细胞毒性。
