Köster H
Arzneimittelforschung. 1980;30(3a):54-57.
A review of applications of chemically synthesized oligodeoxynucleotides to gene technology is given. At first the reader is briefly introduced to the methods of oligodeoxynucleotide synthesis. With selected examples the utilization of synthetic oligodeoxynucleotides is discussed in some detail for sequencing DNA and RNA, for the incorporation of base analogs and sequence specific mutations into nucleic acids of natural origin, for the isolation of specific nucleic acid sequences from biological sources, for the specific inhibition of virus reproduction, as linker molecules for gene cloning experiments and for the total synthesis of signal structures (regulatory sequences) and structural genes. The importance of synthetic oligodeoxynucleotides for molecular biology and gene technology especially is emphasized.
本文综述了化学合成的寡聚脱氧核苷酸在基因技术中的应用。首先向读者简要介绍了寡聚脱氧核苷酸的合成方法。通过具体实例，详细讨论了合成寡聚脱氧核苷酸在DNA和RNA测序、将碱基类似物和序列特异性突变引入天然来源核酸、从生物来源分离特定核酸序列、特异性抑制病毒繁殖、作为基因克隆实验的连接分子以及信号结构（调控序列）和结构基因的全合成等方面的应用。强调了合成寡聚脱氧核苷酸对分子生物学和基因技术的重要性。
