Rich S A
Science. 1981 Aug 14;213(4509):772-5. doi: 10.1126/science.6166984.
Raji cells, a human B lymphoblastoid cell line of Burkitt lymphoma origin, formed lupus inclusions when grown in a medium conditioned by the growth of Raji cells whose DNA thymidine residues had been unifilarly (single-strandedly) substituted with bromodeoxyuridine. Ultracentrifugation of this medium in excess of that required to remove Epstein-Barr virus and all other known mammalian viruses did not prevent the formation of the inclusions, and treatment of the conditioned medium with pronase destroyed the activity. These results demonstrate the presence of a protein that is secreted from bromodeoxyuridine-substituted Raji cells and is capable of inducing nonbromodeoxyuridine-substituted cells to form lupus inclusions. Interferon (100 units per milliliter) was found in the conditioned medium. Inclusions also formed in Raji cells grown in fresh medium supplemented with human leukocyte or fibroblast interferon (100 units per milliliter).
拉吉细胞是一种源自伯基特淋巴瘤的人B淋巴母细胞系，当在由DNA胸腺嘧啶残基被溴脱氧尿苷单链取代的拉吉细胞生长所条件化的培养基中生长时，会形成狼疮包涵体。对该培养基进行超速离心，超过去除爱泼斯坦-巴尔病毒和所有其他已知哺乳动物病毒所需的速度，并未阻止包涵体的形成，用链霉蛋白酶处理条件化培养基会破坏其活性。这些结果表明存在一种从溴脱氧尿苷取代的拉吉细胞分泌的蛋白质，它能够诱导未被溴脱氧尿苷取代的细胞形成狼疮包涵体。在条件化培养基中发现了干扰素（每毫升100单位）。在补充有人白细胞或成纤维细胞干扰素（每毫升100单位）的新鲜培养基中生长的拉吉细胞中也形成了包涵体。
