James R E, Polan C E, Cummins K A
J Dairy Sci. 1981 Jan;64(1):52-61. doi: 10.3168/jds.S0022-0302(81)82528-3.
Ten calves less than 14 h of age (average 8.6 h) were anaesthetized, and the intestine was ligated into segments 10 cm in length at 3-cm intervals beginning 1.8 m anterior of the ileocecal junction and proceeding proximally. Seven treatments were assigned in random order to segments in three successive sections of the small intestine. Segments received 1 ml of viable bacteria of intestinal origin, autoclaved bacteria of intestinal origin, or sterile microbiological broth at zero time; then after 4 h they were injected with iodine-125 labeled gamma-globulin. After an additional 1.5 h, the experiment was ended and uptake assayed. Two treatments measured anaerobic microbial growth after 4 h incubation with 1 ml of either sterile broth or live bacteria culture. Residual [iodine-125] gamma-globulin was measured in segments receiving 1 ml of sterile broth or live bacteria culture with 5.5-h incubation followed by 15-s exposure to labeled gamma-globulin. Uptake was lowest in segments receiving live bacteria as compared to segments receiving sterile inocula. Number of bacteria per gram of tissue was correlated negatively with uptake. Low corticosteroids in serum were associated with low uptake of gamma-globulin.
选取10头出生不到14小时（平均8.6小时）的小牛进行麻醉，从回盲部前方1.8米处开始，以3厘米的间隔将肠管结扎成长度为10厘米的节段，并向近端延伸。将七种处理以随机顺序分配给小肠连续三个节段中的节段。节段在零时间接受1毫升肠道来源的活细菌、肠道来源的高压灭菌细菌或无菌微生物肉汤；然后在4小时后给它们注射碘-125标记的γ-球蛋白。再过1.5小时后，实验结束并测定摄取量。两种处理方法是在与1毫升无菌肉汤或活细菌培养物孵育4小时后测量厌氧微生物生长情况。在接受1毫升无菌肉汤或活细菌培养物并孵育5.5小时，然后暴露于标记的γ-球蛋白15秒的节段中测量残留的[碘-125]γ-球蛋白。与接受无菌接种物的节段相比，接受活细菌的节段摄取量最低。每克组织中的细菌数量与摄取量呈负相关。血清中皮质类固醇水平低与γ-球蛋白摄取量低有关。
