James R E, Polan C E, McGilliard M L
J Dairy Sci. 1979 Sep;62(9):1415-9. doi: 10.3168/jds.S0022-0302(79)83438-4.
Newborn calves deprived of colostrum were used to determine distribution of uptake of gamma-globulin labeled with iodine-125 in small intestine. Ten calves less than 12.5 h of age (average 7 h) were anesthetized, and intestines were exteriorized through an abdominal incision. Intestine was ligated into 10-cm segments, 70 cm apart, beginning at the ileocecal junction and progressing anteriorally, then each segment injected with 100 mg (appoximately 1 microCi) labeled gamma-globulin in 5 ml electrolyte solution, and incubated for 1.5 h. One additional segment was formed adjacent to segments 1, 5, and 10 to assess uptake after .5 h incubation with [iodine-125] gamma-globulin. After prescribed gamma-globulin exposure, segments were excised; then volume of lumen contents, segment weight, and tissue activity were measured. The location of each segment was the percentage of distance from cecum to abomasum. Uptake was milligrams gamma-globulin per gram of segment tissue. Distribution of gamma-globulin uptake after 1.5 h incubation was a cubic function of segment position. Uptake was greatest in a region 15% of the cecum-abomasum distance and declined progressively toward the abomasum. After .5-h incubation with gamma-globulin, regression of uptake on segment position was a quadratic function with greatest uptake at 30% of cecum-abomasum distance. Uptake was greater in segments exposed to gamma-globulin for 1.5 h than .5 h.
利用新生且未摄入初乳的小牛来确定小肠中碘 - 125标记的γ - 球蛋白的摄取分布情况。选取10头年龄小于12.5小时（平均7小时）的小牛进行麻醉，通过腹部切口将小肠引出体外。从小肠回盲部开始，向前每隔70厘米将小肠结扎成10厘米长的节段，然后向每个节段注射100毫克（约1微居里）溶解于5毫升电解质溶液中的标记γ - 球蛋白，并孵育1.5小时。在与[碘 - 125]γ - 球蛋白孵育0.5小时后，在第1、5和10节段旁边额外形成一个节段，以评估摄取情况。在规定的γ - 球蛋白暴露时间后，切除各节段；然后测量肠腔内容物体积、节段重量和组织活性。每个节段的位置是以从盲肠到皱胃的距离百分比来表示。摄取量是以每克节段组织中γ - 球蛋白的毫克数来计算。孵育1.5小时后γ - 球蛋白摄取的分布是节段位置的三次函数。在距盲肠 - 皱胃距离的15%处摄取量最大，向皱胃方向逐渐下降。与γ - 球蛋白孵育0.5小时后，摄取量与节段位置的回归是二次函数，在距盲肠 - 皱胃距离的30%处摄取量最大。与γ - 球蛋白孵育1.5小时的节段摄取量大于孵育0.5小时的节段。
