Wahlström T, Stenman U H, Lundqvist C, Tanner P, Schröder J, Seppälä M
J Histochem Cytochem. 1981 Jul;29(7):864-5. doi: 10.1177/29.7.6167612.
A monoclonal mouse antibody to human chorionic gonadotropin (hCG) was used in a modified unlabeled antibody enzyme-bridge staining method to demonstrate the localization of hCG in normal human placenta, pituitary gland, and six pituitary chromophobe adenomas. Mouse ascitic fluid containing monoclonal antibody could be diluted up to 1:500,000 for detection of hCG in the syncytiotrophoblast, whereas no staining was observed in the pituitary or adenomas even with high antibody concentrations (dilutions from 1:500 upward). Nonspecific background staining was negligible. These results demonstrate that monoclonal antibodies are suitable for immunohistochemical localization of antigens in tissues.
一种针对人绒毛膜促性腺激素（hCG）的单克隆小鼠抗体，被用于一种改良的未标记抗体酶桥染色法，以显示hCG在正常人体胎盘、垂体以及六个垂体嫌色细胞瘤中的定位。含有单克隆抗体的小鼠腹水可稀释至1:500,000用于检测合体滋养层中的hCG，而即使使用高浓度抗体（从1:500向上稀释），在垂体或腺瘤中也未观察到染色。非特异性背景染色可忽略不计。这些结果表明，单克隆抗体适用于组织中抗原的免疫组化定位。
