一种用于评估血清和脑脊液中α2-巨球蛋白水平的显色测定法。

A chromogenic assay for evaluation of alpha 2-macroglobulin level in serum and cerebrospinal fluid.

作者信息

Cullmann W, Dick W

出版信息

J Clin Chem Clin Biochem. 1981 May;19(5):287-90. doi: 10.1515/cclm.1981.19.5.287.

DOI:10.1515/cclm.1981.19.5.287

Abstract

A photometric assay using the chromogenic substrate carbobenzoxy-valyl-glycyl-arginine-p-nitroanilide acetate (CHROMOZYM TRY) for the determination of alpha 2-macroglobulin which binds trypsin as a trypsin-protein complex, thus preserving its amidolytic activity, even in the presence of aprotinin, has been studied in serum as well as in cerebrospinal fluid (CSF). Normal range in sera was found to be mean = 10.2 +/- 3.3 kU/l (male), mean = 10.9 +/- 3.2 kU/l (female), and mean = 11.9 +/- 5.0 U/l in CSF. Within-run precision in sera was ascertained to be C.V. = 2.06%, and in CSF C.V. = 2.15%, respectively. There was a good correlation with immunochemical methods: r = 0.974 in 60 sera (p less than 0.001) and r = 0.976 in 16 pathological samples of CSF (p less than 0.001).

摘要

一种使用生色底物苄氧羰基-缬氨酰-甘氨酰-精氨酸-对硝基苯胺乙酸盐（CHROMOZYM TRY）的光度测定法，用于测定α2-巨球蛋白。α2-巨球蛋白作为胰蛋白酶-蛋白质复合物结合胰蛋白酶，即使在存在抑肽酶的情况下也能保持其酰胺水解活性。该方法已在血清和脑脊液（CSF）中进行了研究。血清中的正常范围为男性平均 = 10.2 +/- 3.3 kU/l，女性平均 = 10.9 +/- 3.2 kU/l，脑脊液中平均 = 11.9 +/- 5.0 U/l。血清中的批内精密度确定为变异系数（C.V.）= 2.06%，脑脊液中C.V. = 2.15%。与免疫化学方法有良好的相关性：60份血清中r = 0.974（p < 0.001），16份脑脊液病理样本中r = 0.976（p < 0.001）。