Jackson D C, Brown L E, White D O
J Gen Virol. 1981 Jan;52(Pt 1):163-8. doi: 10.1099/0022-1317-52-1-163.
The oligosaccharide sidechains attached to the major polypeptide, HA1 of the haemagglutinin of influenza virus were examined for antigenic activity using a solid-phase radioimmunoassay. Cross-reactivity between the HA1 of the different human subtypes was clearly demonstrable with IgG raised against purified virus but was abrogated if anti-carbohydrate antibodies were first removed by passage of the IgG through an immunoadsorbent column containing haemagglutinin (HA) from an unrelated avian influenza strain. Antibodies eluted from the column were found to cross-react with the HA1 of all subtypes tested. 'Host antigen' extracted from chick chorioallantoic membrane and coupled to Sepharose was also able to remove cross-reactive antibodies from antiviral sera, while antibodies raised against host antigen bound to the HA1 isolated from each subtype tested. It is concluded that, although there are qualitative and quantitative differences between the oligosaccharide sidechains of influenza haemagglutinins, the antigenically active sidechains are cross-reactive.
使用固相放射免疫测定法检测了与流感病毒血凝素的主要多肽HA1相连的寡糖侧链的抗原活性。用针对纯化病毒产生的IgG可明显证明不同人类亚型的HA1之间存在交叉反应，但如果通过使IgG通过含有来自无关禽流感毒株的血凝素（HA）的免疫吸附柱首先去除抗碳水化合物抗体，则交叉反应会消除。从该柱洗脱的抗体被发现与所有测试亚型的HA1发生交叉反应。从鸡绒毛尿囊膜中提取并偶联到琼脂糖凝胶上的“宿主抗原”也能够从抗病毒血清中去除交叉反应性抗体，而针对与从每个测试亚型分离的HA1结合的宿主抗原产生的抗体则与之结合。得出的结论是，尽管流感血凝素的寡糖侧链之间存在质和量的差异，但具有抗原活性的侧链是交叉反应性的。
