Bhargava G, Stone W H
Anim Blood Groups Biochem Genet. 1977;8(4):209-15. doi: 10.1111/j.1365-2052.1977.tb01648.x.
The erythrocyte antigen (Ecl) of cattle has been isolated using DEAE-cellulose chromatography, Sephadex gel filtration, and isoelectrofocusing. The purified antigen is free from hemoglobin and migrates as a single protein on polyacrylamide gels. It exhibits an absorption maximum at 278 nm and has a molecular weight of about 100 000; its isoelectric point is 4.95. The antigen has a carbohydrate moeity and loses its activity after oxidation with periodate. The evidence suggests that it is not a common isozyme, but is exact identity remains unknown.
已使用二乙氨基乙基纤维素色谱法、葡聚糖凝胶过滤法和等电聚焦法分离出牛的红细胞抗原（Ecl）。纯化后的抗原不含血红蛋白，在聚丙烯酰胺凝胶上作为单一蛋白质迁移。它在278nm处有最大吸收峰，分子量约为100000；其等电点为4.95。该抗原具有碳水化合物部分，经高碘酸盐氧化后失去活性。有证据表明它不是一种常见的同工酶，但其确切身份仍不清楚。
