曼氏血吸虫感染的血清学诊断：使用含有曼氏血吸虫卵抗原ω1和α1的组分进行酶联免疫吸附测定（ELISA），可提高敏感性和特异性。

Serodiagnosis of human Schistosoma mansoni infections: enhanced sensitivity and specificity in ELISA using a fraction containing S. mansoni egg antigens omega 1 and alpha 1.

作者信息

McLaren M L, Lillywhite J E, Dunne D W, Doenhoff M J

出版信息

Trans R Soc Trop Med Hyg. 1981;75(1):72-9. doi: 10.1016/0035-9203(81)90014-6.

Abstract

Six fractions of Schistosoma mansoni egg homogenate obtained by cation exchange chromatography were tested in an enzyme-linked immunosorbent assay (ELISA) for their efficacy in serodiagnosis of human S. mansoni infections. One cationic fraction (CEF6) containing egg antigens omega 1 and alpha 1, was found to be highly reactive with a S. mansoni positive reference serum pool. 94 of 95 sera from individuals infected with S. mansoni gave a positive reaction with CEF6, and no cross reactivity was obtained with this antigenic fraction and sera from donors infected with heterologous non-schistosome parasites and cases of avian cercarial dermatitis. Only 10 of 165 sera from patients with S. haematobium and three of 10 sera from patients with S. japonicum were positive with S. mansoni egg CEF6. In one group of patients from West Africa, examined both serologically and parasitologically at a six-monthly interval, the measurement of antibody to CEF6 provided a more sensitive diagnostic aid than stool examination.

摘要

通过阳离子交换色谱法获得的曼氏血吸虫卵匀浆的六个组分，在酶联免疫吸附测定（ELISA）中测试了它们在人类曼氏血吸虫感染血清学诊断中的功效。发现一个含有卵抗原ω1和α1的阳离子组分（CEF6）与曼氏血吸虫阳性参考血清库具有高度反应性。95份来自感染曼氏血吸虫个体的血清中有94份与CEF6呈阳性反应，并且该抗原组分与来自感染异源非血吸虫寄生虫的供体血清以及禽尾蚴性皮炎病例血清均未出现交叉反应。165份埃及血吸虫病患者的血清中只有10份以及10份日本血吸虫病患者的血清中有3份对曼氏血吸虫卵CEF6呈阳性反应。在一组来自西非的患者中，每六个月进行一次血清学和寄生虫学检查，结果显示，检测针对CEF6的抗体比粪便检查提供了更敏感的诊断辅助手段。