Dunne D W, Bain J, Lillywhite J, Doenhoff M J
Trans R Soc Trop Med Hyg. 1984;78(4):460-70. doi: 10.1016/0035-9203(84)90061-0.
The immunological properties of CEF6, a cationic fraction of Schistosoma mansoni egg homogenate (SEA) containing two antigens, omega 1 and alpha 1, have been further investigated in two assays, immunoelectrophoresis (IEP) and enzyme immunoassay (ELISA). Although precipitating antibodies to antigen omega 1 were amongst the first to appear in mice with patient infections, IgG reactivity against CEF6 in ELISA trailed somewhat behind that of IgG activity against unfractionated SEA. Specificity of the two antigens in CEF6 to the egg stage of the parasite life-cycle was demonstrated by the failure of immunizations with cercarial or worm antigens to induce antibodies which reacted against either alpha 1 or omega 1 in IEP, or against CEF6 in ELISA. Mice infected with S. mansoni strains derived from geographically distinct areas, including the Caribbean, South America and Africa, produced antibodies which were reactive against CEF6 prepared from eggs of a Puerto Rican S. mansoni strain that had been maintained in the laboratory for many years. Of the different precipitating anti-SEA antibody species induced by the various S. mansoni strains in mice, those reactive against antigen omega 1 appeared to be present in highest titre. Sera from mice chronically infected with S. japonicum and S. haematobium failed to precipitate CEF6 in IEP and were less reactive with CEF6 than with S. mansoni SEA in ELISA. However, similar degrees of ELISA reactivity against S. mansoni CEF6 and SEA were given by sera from mice infected with S. bovis. The results support the notion that the antigens in CEF6 may be useful in the serodiagnosis of schistosomiasis mansoni infections.
曼氏血吸虫卵匀浆（SEA）的阳离子组分CEF6含有两种抗原，即ω1和α1，其免疫特性已在免疫电泳（IEP）和酶免疫测定（ELISA）这两种检测方法中得到进一步研究。尽管针对抗原ω1的沉淀抗体是感染患者的小鼠体内最早出现的抗体之一，但在ELISA中，针对CEF6的IgG反应性略落后于针对未分级SEA的IgG活性。通过用尾蚴或虫体抗原进行免疫未能诱导出在IEP中与α1或ω1反应、或在ELISA中与CEF6反应的抗体，证明了CEF6中的两种抗原对寄生虫生命周期卵阶段的特异性。感染了来自包括加勒比地区、南美洲和非洲等地理上不同区域的曼氏血吸虫菌株的小鼠产生的抗体，与从在实验室中保存多年的波多黎各曼氏血吸虫菌株的卵制备的CEF6发生反应。在由各种曼氏血吸虫菌株在小鼠体内诱导产生的不同沉淀抗SEA抗体种类中，针对抗原ω1反应的抗体似乎滴度最高。慢性感染日本血吸虫和埃及血吸虫的小鼠血清在IEP中未能沉淀CEF6，并且在ELISA中与CEF6的反应性低于与曼氏血吸虫SEA的反应性。然而，感染牛血吸虫的小鼠血清对曼氏血吸虫CEF6和SEA的ELISA反应性程度相似。这些结果支持了CEF6中的抗原可能有助于曼氏血吸虫病感染血清诊断的观点。
