Indo K, Miyaji H
Gan. 1981 Feb;72(1):123-31.
The effect of skin tumor promote, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), and related phorbol esters on primary diploid and four established, i.e. two aneuploid premalignant and two malignant, lines of fetal rat epidermal cells wee analyzed. TPA quantitatively or qualitatively affected the keratinization processes of cultured epidermal cells in the concentration range of 10 - 1,000 ng/ml. Its inhibitory effect on keratinization was obvious in the lamellar keratinization processes of aneuploid premalignant epidermal cells. With a few exceptions, the inhibition was reversible. However, TPA lethally damaged the primary diploid epidermal cells in this range of concentration. TPA did not inhibit the keratinization of malignant epidermal cells but destroyed the polarity in their keratinization processes.
分析了皮肤肿瘤促进剂12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）及相关佛波酯对原代二倍体和四种已建立的（即两种非整倍体癌前和两种恶性）胎鼠表皮细胞系的影响。TPA在10 - 1000 ng/ml浓度范围内对培养的表皮细胞角质化过程有定量或定性影响。其对角质化的抑制作用在非整倍体癌前表皮细胞的板层角质化过程中很明显。除少数例外，这种抑制是可逆的。然而，在此浓度范围内TPA会致死性损伤原代二倍体表皮细胞。TPA不抑制恶性表皮细胞的角质化，但会破坏其角质化过程中的极性。
