Nagayoshi S, Imai M, Tsutsui Y, Saga S, Takahashi M, Hoshino M
Gan. 1981 Feb;72(1):98-103.
Ninety-two sera from patients with breast cancer, 42 sera from patients with neoplastic diseases other than breast cancer and 59 sera from apparently healthy women were examined by means of the immune adherence hemagglutination (IAHA) test using purified mouse mammary tumor virus (MMTV) fron RII mouse milk. It was found that 36.4% (34/96) of the sera from breast cancer patients, 7.1% (3/42) of the sera from patients with other neoplastic diseases and 5.1% (3/59) of the sera from apparently healthy women showed a positive reaction. Among the IAHA positive sera from breast cancer patients, 82.9% (29/35) showed a titer of more than 1:16. On the other hand, none of the positive sera from patients with cancers other than breast cancer showed a titer of more than 1:16. The sera from 4 breast cancer patients, which showed a positive reaction with RII MMTV in the IAHA test, were tested to examine the specificity of the reaction by using milk samples from sources other than RII mice, including C57BL mice, dogs, cattle and humans. None of the 4 sera showed a positive reaction with milk samples from sources other than the RIII mouse.
使用从RII小鼠乳汁中提取的纯化小鼠乳腺肿瘤病毒（MMTV），通过免疫黏附血凝试验（IAHA）对92份乳腺癌患者血清、42份非乳腺癌肿瘤疾病患者血清以及59份貌似健康女性的血清进行检测。结果发现，乳腺癌患者血清中有36.4%（34/96）呈阳性反应，其他肿瘤疾病患者血清中有7.1%（3/42）呈阳性反应，貌似健康女性血清中有5.1%（3/59）呈阳性反应。在乳腺癌患者IAHA阳性血清中，82.9%（29/35）的滴度高于1:16。另一方面，非乳腺癌肿瘤疾病患者的阳性血清中无一例滴度高于1:16。对在IAHA试验中与RII MMTV呈阳性反应的4例乳腺癌患者血清，使用包括C57BL小鼠、狗、牛和人类等RII小鼠以外来源的乳汁样本检测反应的特异性。这4份血清中无一例与RIII小鼠以外来源的乳汁样本呈阳性反应。
