Hook W A, Siraganian R P
Immunology. 1981 Aug;43(4):723-31.
Allergen-activated human basophils require Ca2+ for the in vitro release of histamine. This study evaluated the importance of anions, cations and osmotic pressure on histamine release from human basophils. All media contained 1 mM Ca2+ and various salts or sugars were used to replace the NaCl-KCl of the control medium. Permeant anions supported release of histamine with the following order of activities: acetate - greater than Br-, I-, greater than Cl- greater than SO42-. When monovalent cations of the standard medium were replaced, RbCl, CsCl, KCl or NaCl gave results identical to the NaCl-KCl control medium; choline chloride usually enhanced while LiCl inhibited release. Finally, when sugar solutions were used to replace monovalent anions and cations of the standard medium: mannitol, lactose, sucrose, sorbitol, fructose and glucose each supported histamine release. Increasing the osmolarity of the medium by adding additional NaCl-KCl enhanced the ability of suboptimal concentrations of ragweed antigen E or anti-IgE to activate and to release histamine from leucocytes. The results indicate that histamine from leucocytes. The results indicate that histamine release from basophils requires only Ca2+ and do not support the "chemiosmotic swelling' hypothesis of secretion for these cells.
变应原激活的人嗜碱性粒细胞在体外释放组胺需要Ca2+。本研究评估了阴离子、阳离子和渗透压对人嗜碱性粒细胞组胺释放的重要性。所有培养基均含有1 mM Ca2+，并使用各种盐或糖来替代对照培养基中的NaCl-KCl。渗透性阴离子支持组胺释放，其活性顺序如下：醋酸根＞Br-、I-＞Cl-＞SO42-。当标准培养基中的单价阳离子被替代时，RbCl、CsCl、KCl或NaCl的结果与NaCl-KCl对照培养基相同；氯化胆碱通常会增强释放，而LiCl则会抑制释放。最后，当使用糖溶液替代标准培养基中的单价阴离子和阳离子时：甘露醇、乳糖、蔗糖、山梨醇、果糖和葡萄糖均支持组胺释放。通过添加额外的NaCl-KCl来增加培养基的渗透压，可增强次优浓度的豚草抗原E或抗IgE激活白细胞并从中释放组胺的能力。结果表明白细胞释放组胺的情况。结果表明嗜碱性粒细胞释放组胺仅需要Ca2+，并不支持这些细胞分泌的“化学渗透肿胀”假说。
