环磷酰胺和6-巯基嘌呤对豚鼠IgG1和IgG2免疫反应的影响。
The influence of cyclophosphamide and 6-mercaptopurine on the IgG1 and IgG2 immune response in guinea-pigs.
作者信息
Drössler K, Klima F, Ambrosius H
出版信息
Immunology. 1981 Sep;44(1):61-6.
The purpose of this study was to investigate the effect of cyclophosphamide (CY) and 6-mercaptopurine (6-MP) on the production of the two IgG antibody subclasses in guinea-pigs. Following the intradermal application of dinitrophenylated bovine gamma globulin (DNP20-BGG) emulsified in Freund's incomplete adjuvant (FIA) each animal was given CY or 6-MP, 10 mg per kg of body weight per day on days 1--7, and the DNP- and BGG-specific anaphylactic (IgG1) and lytic (IgG2) antibodies were determined. Only treatment with CY resulted in a significantly-depressed IgG2 anti-DNP immune response; 6-MP was not suppressive and caused a prolonged IgG2 anti-DNP production. Both substances showed a stimulatory effect on IgG1 anti-DNP antibody formation. The carrier-specific IgG1 and IgG2 antibody levels were suppressed completely by CY but unaffected by 6-MP. After a booster injection of 1 mg BGG on day 86 the animals in the CY-treated group were not able to mount a secondary IgG2 anti-BGG immune response. In contrast, IgG1 anti-BGG production was strong and uninfluenced by CY treatment.
本研究的目的是探讨环磷酰胺（CY）和6-巯基嘌呤（6-MP）对豚鼠两种IgG抗体亚类产生的影响。在皮内注射用弗氏不完全佐剂（FIA）乳化的二硝基苯基化牛γ球蛋白（DNP20-BGG）后，每只动物于第1 - 7天每天给予CY或6-MP，剂量为每千克体重10毫克，并测定DNP特异性和BGG特异性的过敏反应性（IgG1）和溶细胞性（IgG2）抗体。仅用CY治疗导致IgG2抗DNP免疫反应显著降低；6-MP没有抑制作用，反而使IgG2抗DNP产生时间延长。两种物质对IgG1抗DNP抗体形成均有有有刺激作用。载体特异性IgG1和IgG2抗体水平被CY完全抑制，但不受6-MP影响。在第86天给予1毫克BGG加强注射后，CY治疗组的动物无法产生继发性IgG2抗BGG免疫反应。相反，IgG1抗BGG产生强烈，且不受CY治疗的影响。
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