豚鼠IgG1和IgG2中Fab糖基化的不对称性
Asymmetric Fab glycosylation in guinea-pig IgG1 and IgG2.
作者信息
Malan Borel I, Gentile T, Angelucci J, Margni R A, Binaghi R A
机构信息
Departamento de Microbiologia, Immunologia y Biotecnologia, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
出版信息
Immunology. 1990 Jul;70(3):281-3.
The presence of asymmetric antibody molecules has been investigated in both IgG1 and IgG2 subclasses of guinea-pig immunoglobulins. It was found that about 20% of the IgG1 and 10% of the IgG2 were of asymmetric type. The proportion was essentially the same in the sera of normal animals, animals hyperimmunized with dinitrophenyl-bovine gamma globulin (DNP-BGG) and Freund's adjuvant, and animals infected with Trichinella spiralis. In the case of animals immunized with DNP-BGG, no differences were observed in the proportion of asymmetric molecules between the specific antibodies and the IgG not specific for the immunizing antigen. It is concluded that the asymmetric glycosylation occurs to a different extent in each subclass and that it is not affected by the antigen specificity of the antibodies studied.
已对豚鼠免疫球蛋白的IgG1和IgG2亚类中不对称抗体分子的存在情况进行了研究。发现约20%的IgG1和10%的IgG2为不对称型。在正常动物、用二硝基苯基 - 牛γ球蛋白（DNP - BGG）和弗氏佐剂进行超免疫的动物以及感染旋毛虫的动物血清中，该比例基本相同。在用DNP - BGG免疫的动物中，特异性抗体与对免疫抗原无特异性的IgG之间，不对称分子的比例未观察到差异。得出的结论是，每个亚类中不对称糖基化的程度不同，并且它不受所研究抗体的抗原特异性影响。
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