Calvert J E, Ling N R, Jefferis R
Immunology. 1981 Sep;44(1):89-95.
Proteolysis of lymphocyte surface membrane immunoglobulin (SIg) by trypsin, papain and pronase was investigated. Cleavage in the hinge region resulting in removal of Fab delta was distinguished from removal of the whole SIgD molecule using antisera specific for c delta l and Fc delta. Using the sensitive direct rosette assay SIgD determinants were detected after digestion under conditions previously thought to remove IgD from the membrane. By increasing digestion times is was shown that whilst papain and pronase remove the whole IgD molecule, Fc delta but not Fab delta determinants survive trypsin. The implications of the results for structural differences between membrane and serum IgD and for models of insertion of SIgD into the plasma membrane are discussed.
研究了胰蛋白酶、木瓜蛋白酶和链霉蛋白酶对淋巴细胞表面膜免疫球蛋白（SIg）的蛋白水解作用。使用针对cδ1和Fcδ的抗血清，区分了铰链区的切割导致Fabδ去除与整个SIgD分子的去除。使用灵敏的直接玫瑰花结试验，在先前认为可从膜上去除IgD的条件下消化后检测到了SIgD决定簇。通过延长消化时间表明，虽然木瓜蛋白酶和链霉蛋白酶可去除整个IgD分子，但Fcδ决定簇而非Fabδ决定簇在胰蛋白酶处理后仍存在。讨论了这些结果对膜IgD和血清IgD结构差异以及SIgD插入质膜模型的意义。
