Suciu-Foca N, Godfrey M, Khan R
Hum Immunol. 1981 Jul;2(4):295-304. doi: 10.1016/0198-8859(81)90031-8.
The primed lymphocyte test (PLT) detects gene products of the HLA-D-DR region which activate the secondary (memory) response of MLC stimulated T cells. In the present study attempts were made to determine whether different HLA-D alleles associated with the same DR, such as DR1, 2, and 4 can be discriminated by PLT typing. PLTs were generated by using, as responders and primary MLC stimulators, HLA-D different HTCs which shared all DR groups (major DR, supertypic MT and second locus MB) or only the MT or MB groups. As secondary stimulators, lymphocytes from an HLA-D selected panel of 72 individuals were used. PLTs raised in DR identical responder-primary stimulator combinations were able to discriminate between the different HLA-D antigens associated with the same DR. In contrast, when priming was performed in combinations differing for the major DR group, the restimulation response was highly associated with the DR specificity of the primary stimulator, regardless of whether or not this was compatible with the responding HTC for the MT or MB groups. This data indicate that the specificity of primed lymphocytes largely depends on the combinations used for priming and that the memory response can be activated by both HLA-D and DR antigens. The dissociation of HLA-D from DR by PLT typing might provide a useful tool for further analysis of this HLA region.
致敏淋巴细胞试验（PLT）可检测HLA - D - DR区域的基因产物，这些产物可激活混合淋巴细胞培养（MLC）刺激的T细胞的二次（记忆）反应。在本研究中，我们试图确定是否可以通过PLT分型来区分与相同DR相关的不同HLA - D等位基因，如DR1、DR2和DR4。PLT是通过使用HLA - D不同的HTC细胞作为反应细胞和初次MLC刺激细胞来产生的，这些HTC细胞共享所有DR组（主要DR、超型MT和第二位点MB）或仅共享MT或MB组。作为二次刺激细胞，使用了来自72名个体的HLA - D选择面板的淋巴细胞。在DR相同的反应细胞 - 初次刺激细胞组合中产生的PLT能够区分与相同DR相关的不同HLA - D抗原。相比之下，当在主要DR组不同的组合中进行致敏时，再刺激反应与初次刺激细胞的DR特异性高度相关，无论其是否与反应HTC细胞的MT或MB组兼容。该数据表明，致敏淋巴细胞的特异性很大程度上取决于用于致敏的组合，并且记忆反应可由HLA - D和DR抗原激活。通过PLT分型将HLA - D与DR分离可能为进一步分析该HLA区域提供有用的工具。
