大肠杆菌K-12中精氨酸特异性信使核糖核酸的代谢
Metabolism of arginine-specific messenger ribonucleic acid in Escherichia coli K-12.
作者信息
Natter W, Sens D, James E
出版信息
J Bacteriol. 1977 Jul;131(1):214-23. doi: 10.1128/jb.131.1.214-223.1977.
Abstract
Ribonucleic acid-deoxyribonucleic acid (RNA-DNA) hybridization was employed for the determination of the level of messenger RNA (mRNA) transcribed from seven of the nine genes of the arginine regulon of Escherichia coli K-12. The quantity of RNA complexing with each of the separated DNA strands of the argA, argF, argE, and argCBH operons carried on specialized transducing phages was measured. The derepressed:repressed ratio of mRNA formed in vivo was found to vary between about 3 and 4 when measured by hybridization to DNA isolated from specialized transducing phages carrying the argA, argE, argCBH, argF, and argI operons.
摘要
采用核糖核酸 - 脱氧核糖核酸（RNA - DNA）杂交技术来测定大肠杆菌K - 12精氨酸调节子九个基因中七个基因转录的信使核糖核酸（mRNA）水平。测定了与携带在专门转导噬菌体上的argA、argF、argE和argCBH操纵子的每条分离DNA链结合的RNA量。当通过与携带argA、argE、argCBH、argF和argI操纵子的专门转导噬菌体分离的DNA杂交来测量时，发现体内形成的mRNA的去阻遏：阻遏比率在约3至4之间变化。
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