基因片段的自动化合成
Automated synthesis of gene fragments.
作者信息
Alvarado-Urbina G, Sathe G M, Liu W C, Gillen M F, Duck P D, Bender R, Ogilvie K K
出版信息
Science. 1981 Oct 16;214(4518):270-4. doi: 10.1126/science.6169150.
PMID:6169150
Abstract
The DNA/RNA Synthesizer provides a complete and automated procedure for the synthesis of DNA sequences. Each base unit is added in a 30-minute cycle, permitting a tetradecamer to be constructed in 6 1/2 hours. The complete procedure is described, including a practical procedure for isolation and purification of the desired DNA sequence.
摘要
DNA/RNA合成仪为DNA序列的合成提供了一个完整的自动化程序。每个碱基单元以30分钟的周期添加，使得一个十四聚体能够在6个半小时内构建完成。文中描述了完整的程序，包括分离和纯化所需DNA序列的实际操作步骤。
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