Jacobs S C, Lawson R K
Urol Int. 1981;36(3):194-8. doi: 10.1159/000280411.
Total prostatectomies were performed in 10 young male cadaver renal donors and explant cultures were prepared in Falcon flasks. Growth of a monolayer of predominantly epithelial cells occurred rapidly (latent time 3-5 days) and the flasks became confluent within 15-17 days. The monolayers grew well even when a needle was used to circumscribe and remove the explants. Passaging of monolayer cells by seeding at a density of 5 x 10(5) resulted in the vigorous growth of epithelial cells. However, the cultures were eventually overgrown by fibroblasts. Similar explant cultures of benign prostatic hyperplasia or prostatic carcinoma demonstrated a longer latent time, rarely attained confluence, died after explant removal, and could not be passaged.
对10名年轻男性尸体肾供体进行了全前列腺切除术，并在Falcon培养瓶中制备了外植体培养物。主要为上皮细胞的单层细胞迅速生长（潜伏期3 - 5天），培养瓶在15 - 17天内长满。即使使用针头划界并取出外植体，单层细胞仍生长良好。以5×10⁵的密度接种进行单层细胞传代培养，上皮细胞生长旺盛。然而，最终培养物被成纤维细胞过度生长。良性前列腺增生或前列腺癌的类似外植体培养显示出更长的潜伏期，很少达到汇合状态，在外植体取出后死亡，且无法传代。
