Goeddel D V, Yelverton E, Ullrich A, Heyneker H L, Miozzari G, Holmes W, Seeburg P H, Dull T, May L, Stebbing N, Crea R, Maeda S, McCandliss R, Sloma A, Tabor J M, Gross M, Familletti P C, Pestka S
Nature. 1980 Oct 2;287(5781):411-6. doi: 10.1038/287411a0.
A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid signal peptide followed by an interferon polypeptide of 165 amino acids. An expression plasmid was constructed which permits the synthesis in E. coli of 2.5 x 10(8) units of interferon per litre of culture. This LeIF protected squirrel monkeys from lethal encephalomyocarditis virus infection.
人工合成人白细胞干扰素cDNA，将其插入载体pBR322，并在大肠杆菌中克隆。该DNA序列编码一个由23个氨基酸组成的信号肽，其后是一个由165个氨基酸组成的干扰素多肽。构建了一种表达质粒，可使大肠杆菌在每升培养物中合成2.5×10⁸单位的干扰素。这种人白细胞干扰素可保护松鼠猴免受致死性脑心肌炎病毒感染。
