大肠杆菌产生乙型肝炎病毒具有免疫活性的表面抗原。
Production of immunologically active surface antigens of hepatitis B virus by Escherichia coli.
作者信息
MacKay P, Pasek M, Magazin M, Kovacic R T, Allet B, Stahl S, Gilbert W, Schaller H, Bruce S A, Murray K
出版信息
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4510-4. doi: 10.1073/pnas.78.7.4510.
Abstract
Several plasmids have been constructed which direct the synthesis of hepatitis B virus surface antigens in Escherichia coli either as the native polypeptide or fused to other plasmid encoded polypeptides. When injected into rabbits, extracts from bacteria carrying some of these plasmids induced the synthesis of antibodies to the antigens even though the extracts did not give satisfactory positive results in radioimmunoassay for them. Either the NH2-terminal segment or the COOH-terminal segment of the surface antigens alone was sufficient to elicit the immune response, but antibodies against the two segments showed different specificities. The results emphasize the value of an in vivo assay for the presence of antigens in crude cell extracts and illustrate the feasibility of this type of screening with laboratory animals.
摘要
已经构建了几种质粒，它们可指导大肠杆菌中乙型肝炎病毒表面抗原的合成，合成形式既可以是天然多肽，也可以与其他质粒编码的多肽融合。将携带其中一些质粒的细菌提取物注射到兔子体内后，可诱导抗体合成以对抗这些抗原，尽管这些提取物在针对它们的放射免疫测定中未给出令人满意的阳性结果。单独的表面抗原的氨基末端片段或羧基末端片段足以引发免疫反应，但针对这两个片段的抗体表现出不同的特异性。这些结果强调了在粗细胞提取物中对抗原进行体内检测的价值，并说明了用实验动物进行此类筛选的可行性。
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