真核信号序列在大肠杆菌中转运胰岛素抗原。
Eukaryotic signal sequence transports insulin antigen in Escherichia coli.
作者信息
Talmadge K, Stahl S, Gilbert W
出版信息
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3369-73. doi: 10.1073/pnas.77.6.3369.
Abstract
We made a series of plasmids with unique Pst restriction sites within or near the DNA that encodes the penicillinase signal sequence. Inserted DNA can be read in all three frames both within and immediately after the signal sequence. We cloned Pst-terminated DNA copies of the structural information for rat proinsulin and preproinsulin into these plasmids, forming a large number of hybrid penicillinase (bacterial) and insulin (eukaryotic) signal sequences. We then compared the levels of insulin antigen in the Escherichia coli periplasm with those inside the cells. We conclude that either the bacterial or the eukaryotic signal is sufficient to transport rat insulin antigen into the periplasmic space.
摘要
我们构建了一系列质粒，这些质粒在编码青霉素酶信号序列的DNA内部或附近具有独特的Pst限制性酶切位点。插入的DNA在信号序列内部以及信号序列之后都可以在所有三个阅读框中进行阅读。我们将大鼠胰岛素原和前胰岛素原结构信息的Pst末端DNA拷贝克隆到这些质粒中，形成了大量杂交的青霉素酶（细菌）和胰岛素（真核）信号序列。然后我们比较了大肠杆菌周质中胰岛素抗原的水平与细胞内胰岛素抗原的水平。我们得出结论，细菌信号或真核信号都足以将大鼠胰岛素抗原转运到周质空间。
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