Wallace D M, Kay J E
Biosci Rep. 1981 Jul;1(7):539-45. doi: 10.1007/BF01116302.
The stability of cytoplasmic poly(A)-containing mRNA in unstimulated pig lymphocytes has been determined by [3H]poly(U) hybridization after actinomycin D treatment. This approach has shown that the majority of the mRNA is much more unstable than has been suggested by earlier pulse-chase experiments, and has a half-life of between 1 and 2 h.
在用放线菌素D处理后，通过[3H]聚尿嘧啶核苷杂交法测定了未受刺激的猪淋巴细胞中含细胞质多聚腺苷酸的mRNA的稳定性。该方法表明，大多数mRNA比早期脉冲追踪实验所显示的要不稳定得多，其半衰期在1至2小时之间。
