Pisarev V M, Stukalov S V
Biull Eksp Biol Med. 1981 Aug;92(8):61-3.
CBA mice were immunized with sheep red blood cells (SRBC) to obtain immune spleen cells (ISc) which were used to suppressor cells. Administration of ISC to intact syngeneic recipients on the immunization day led to a more powerful suppression of the immune response as compared to that seen one day after antigen injection. Four days after immunization the animals' immune response was not liable to be suppressed. ISC extract possessed similar effects with respect to the immune response of normal spleen cells which were transplanted to the cyclophosphamide-treated recipients. The immune response of spleen cells from mice immunized with SRBC in a dose of 10(6) was less liable to be suppressed. Hyperimmune spleen cells from donors immunized with SRC in a dose of 10(9) were insensitive to ISC or to the extract. Experiments with the use of adoptive transfer of a mixture of immune and intact T- and B-cells have disclosed that B-cells from hyperimmune donors were resistant to suppression. Therefore, B-lymphocytes are the most probable target cells exposed to T-suppressors in the given system. The mechanism is discussed of the selective effect of T-suppressors on B-cells in the course of the immune response development during immunization with high doses of antigen.
用绵羊红细胞（SRBC）免疫CBA小鼠以获得免疫脾细胞（ISc），这些细胞用作抑制细胞。在免疫当天将ISc给予同基因的完整受体，与抗原注射一天后相比，对免疫反应的抑制作用更强。免疫后四天，动物的免疫反应不易被抑制。ISC提取物对移植到经环磷酰胺处理的受体的正常脾细胞的免疫反应具有类似作用。用10(6)剂量的SRBC免疫的小鼠的脾细胞免疫反应较不易被抑制。用10(9)剂量的SRC免疫的供体的超免疫脾细胞对ISC或提取物不敏感。使用免疫和完整的T细胞与B细胞混合物进行过继转移的实验表明，来自超免疫供体的B细胞对抑制有抗性。因此，在给定系统中，B淋巴细胞是最可能受到T抑制细胞作用的靶细胞。讨论了在高剂量抗原免疫期间免疫反应发展过程中T抑制细胞对B细胞的选择性作用机制。
