Rawdon B B, Andrew A
Cell Tissue Res. 1981;220(2):279-92. doi: 10.1007/BF00210509.
The distribution of gastrin-, cholecystokinin-, glucagon-, secretin-, vasoactive intestinal polypeptide-, substance P-, bombesin-, neurotensin-, motilin-, somatostatin- and avian pancreatic polypeptide-like cells, demonstrated by indirect immunocytochemistry, was studied in samples from the following regions: proventriculus, gizzard, pylorus, duodenum, upper and lower ileum, caeca and rectum. The pylorus is particularly rich in gastrin-, neurotensin- and somatostatin-like cells. No cells immunoreactive for gastric inhibitory polypeptide or insulin were detected. In a number of instances the same cells were found to stain with antisera raised to different gut peptides. This happened with antisera detecting gastrin- and neurotensin-like cells, with secretin, vasoactive intestinal polypeptide, glucagon and substance P. The possibility that antigenic determinants to more than one peptide are contained in certain endocrine-like cells is considered.
通过间接免疫细胞化学方法，对来自以下区域的样本进行了研究，以观察胃泌素、胆囊收缩素、胰高血糖素、促胰液素、血管活性肠肽、P物质、蛙皮素、神经降压素、胃动素、生长抑素和禽胰多肽样细胞的分布情况：腺胃、肌胃、幽门、十二指肠、回肠上段和下段、盲肠和直肠。幽门中胃泌素、神经降压素和生长抑素样细胞特别丰富。未检测到对胃抑制多肽或胰岛素呈免疫反应的细胞。在许多情况下，发现同一细胞对针对不同肠道肽产生的抗血清染色。胃泌素和神经降压素样细胞、促胰液素、血管活性肠肽、胰高血糖素和P物质的抗血清都出现了这种情况。人们考虑了某些内分泌样细胞中可能含有不止一种肽的抗原决定簇的可能性。
