Raine C S, Johnson A B, Marcus D M, Suzuki A, Bornstein M B
J Neurol Sci. 1981 Oct;52(1):117-31. doi: 10.1016/0022-510x(81)90140-4.
Myelinated cultures of mouse spinal cord have been exposed to sera raised in rabbits against whole white matter (anti-WM), myelin basic protein (anti-MBP) and galactocerebroside (anti-GC), the major glycolipid of CNS myelin, to determine which factor in central nervous system (CNS) tissue in vitro is the target of serum demyelinating and myelin swelling antibodies. The sera were tested by radioimmunoassay for activity against MBP and against GC and were also specifically absorbed with MBP, GC and control antigens. Studies were also performed with and without active complement. The findings show that demyelination and myelin swelling in vitro are caused by antibodies against GC and not against MBP. Ultrastructurally, the effects of anti-WM and anti-GC sera with and without complement were indistinguishable. This study demonstrates that GC is a major target in antibody-mediated demyelination.
将小鼠脊髓的有髓鞘培养物暴露于用兔制备的针对全白质（抗-WM）、髓鞘碱性蛋白（抗-MBP）和半乳糖脑苷脂（抗-GC，中枢神经系统髓鞘的主要糖脂）产生的血清中，以确定体外中枢神经系统（CNS）组织中的哪种因子是血清脱髓鞘和髓鞘肿胀抗体的靶标。通过放射免疫测定法检测血清对MBP和GC的活性，并且还用MBP、GC和对照抗原进行特异性吸收。还在有活性补体和无活性补体的情况下进行了研究。研究结果表明，体外脱髓鞘和髓鞘肿胀是由抗GC抗体而非抗MBP抗体引起的。在超微结构上，有补体和无补体的抗-WM和抗-GC血清的作用无法区分。这项研究表明，GC是抗体介导的脱髓鞘的主要靶标。
