Robinson A D, Boyden K N, Hendrickson S M, Muirden K D
J Rheumatol. 1981 Jul-Aug;8(4):547-54.
Proteolytic enzyme inhibitor activity has been measured in the rheumatoid knee by 2 different techniques. The inactivation rate of trypsin injected into the joint in 13 patients was compared with synovial fluid levels of alpha-1-antitrypsin (apha 1AT), alpha-2-macroglobulin (alpha 2M) and trypsin inhibitory capacity (TIC). The lack of correlation and the fact that an inverse relationship was shown between the T1/2 to inactivation and joint damage suggests that additional mechanisms are involved in the inactivation of destructive enzymes. Serum levels of alpha 1AT and TIC were elevated in 36 rheumatoid arthritis (RA) patients when compared with control values and RA synovial fluid inhibitor levels elevated by comparison with osteoarthritic figures. The synovial fluid alpha 1AT and TIC correlated with the activity of the lysosomal enzyme beta-acetyl glucosaminidase (beta AGA) but only trace amount of neutral protease activity was detected probably because of the large concentration of inhibitors present. The deficient Pi phenotype MZ was rarely encountered.
已通过两种不同技术测定了类风湿性膝关节中的蛋白水解酶抑制剂活性。将13例患者关节内注射胰蛋白酶的失活率与滑膜液中α-1-抗胰蛋白酶（α1AT）、α-2-巨球蛋白（α2M）和胰蛋白酶抑制能力（TIC）的水平进行了比较。缺乏相关性以及失活半衰期与关节损伤之间呈反比关系这一事实表明，破坏酶的失活涉及其他机制。与对照值相比，36例类风湿性关节炎（RA）患者的血清α1AT和TIC水平升高，与骨关节炎数据相比，RA滑膜液抑制剂水平升高。滑膜液α1AT和TIC与溶酶体酶β-乙酰氨基葡萄糖苷酶（βAGA）的活性相关，但可能由于存在大量抑制剂，仅检测到微量的中性蛋白酶活性。罕见Pi表型MZ缺陷。
