果蝇无细胞提取物中克隆的tRNA和5S RNA基因的转录
Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract.
作者信息
Dingermann T, Sharp S, Appel B, DeFranco D, Mount S, Heiermann R, Pongs O, Söll D
出版信息
Nucleic Acids Res. 1981 Aug 25;9(16):3907-18. doi: 10.1093/nar/9.16.3907.
Abstract
We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts.
摘要
我们描述了从果蝇Kc细胞制备无细胞提取物的方法，该提取物可用于多种克隆的真核RNA聚合酶III基因的转录。该提取物具有较低的RNA加工核酸酶活性，因此获得的主要产物是初级转录本。
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