在同源无细胞提取物中对黑腹果蝇克隆的转运RNA基因进行转录。

Rajput B, Duncan L, DeMille D, Miller R C, Spiegelman G B

Nucleic Acids Res. 1982 Oct 25;10(20):6541-50. doi: 10.1093/nar/10.20.6541.

Cloned Drosophila melanogaster tRNA genes have been transcribed in a homologous cell-free extract isolated from a Schneider II cell line. The major product of the reaction is a tRNA precursor which is processed to a tRNA sized species. The kinetics of transcription has been followed for 5 different valine tRNA gene clones. The results demonstrate formation of stable transcription complex with at least two kinetic steps. While the rate of formation of the transcription complex is similar to different clones, the ultimate rate of transcription varies dramatically. Comparison of the DNA sequence of the tRNA genes suggests that rate determining nucleotides lie outside the canonical tRNA split-internal promoters.

克隆的黑腹果蝇tRNA基因已在从施耐德II细胞系分离的同源无细胞提取物中进行转录。该反应的主要产物是一种tRNA前体，它被加工成tRNA大小的物种。已对5个不同的缬氨酸tRNA基因克隆的转录动力学进行了跟踪。结果表明形成了具有至少两个动力学步骤的稳定转录复合物。虽然转录复合物的形成速率对不同克隆来说是相似的，但最终转录速率却有很大差异。tRNA基因的DNA序列比较表明，决定速率的核苷酸位于典型的tRNA分裂型内部启动子之外。