Abreu S L, Cannella M S, Hanke J
Microbios. 1981;30(121-122):181-90.
Interferon derived from rat fibroblasts (RFA-1) was pH 2 stable and heat labile. Denaturants such as SDS and urea inactivated this interferon but beta-mercaptoethanol alone did not. IFN yield after Newcastle disease virus was multiplicity of infection (moi) dependent. Optimal induction was obtained with an moi of 10. RFA-1 cells produced small amounts of IFN (less than or equal to 10(1.5) U/ml) when exposed to varying amounts of poly(rI): poly(rC). Addition of DEAE-dextran and/or pretreatment with IFN did not change this response. Pretreatment resulted in increased interferon production, priming, but on the average it was only about a 2-fold increase. However, the kinetics of interferon production were altered by pretreatment with IFN whether there was an increase in production or not. Both IFN synthesis and production peaks were detected 2-4 h earlier in IFN-pretreated RFA-1 cells. Rat IFN exhibited a great deal of cross-species activity. It is 100-300% cross-reactive on mouse (L929B and 3T3) cells, and 5-10% active on guinea pig fibroblasts, human (GM 2767) and bovine (MDBK) cells. These cells were more sensitive to rat IFN than Jensen sarcoma cells (a rat tumour cell line). Neither chicken embryo fibroblasts nor monkey (Vero) cells developed an antiviral state when exposed to rat IFN.
源自大鼠成纤维细胞的干扰素（RFA - 1）在pH 2条件下稳定，但对热不稳定。SDS和尿素等变性剂可使这种干扰素失活，但单独的β - 巯基乙醇则不会。新城疫病毒感染后干扰素的产量取决于感染复数（moi）。当moi为10时可获得最佳诱导效果。当暴露于不同量的聚（rI）：聚（rC）时，RFA - 1细胞产生少量的干扰素（小于或等于10(1.5) U/ml）。添加DEAE - 葡聚糖和/或用干扰素预处理不会改变这种反应。预处理导致干扰素产量增加，即引发作用，但平均而言仅增加约2倍。然而，无论产量是否增加，用干扰素预处理都会改变干扰素产生的动力学。在干扰素预处理的RFA - 1细胞中，干扰素合成和产生的峰值均提前2 - 4小时出现。大鼠干扰素表现出大量的种间活性。它在小鼠（L929B和3T3）细胞上具有100 - 300%的交叉反应性，在豚鼠成纤维细胞、人（GM 2767）和牛（MDBK）细胞上具有5 - 10%的活性。这些细胞对大鼠干扰素比Jensen肉瘤细胞（一种大鼠肿瘤细胞系）更敏感。当暴露于大鼠干扰素时，鸡胚成纤维细胞和猴（Vero）细胞均未形成抗病毒状态。
